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. 1987 May;84(10):3334–3338. doi: 10.1073/pnas.84.10.3334

Gene tandem-mediated selection of coliphage λ-receptive Agrobacterium, Pseudomonas, and Rhizobium strains

Robert A Ludwig 1
PMCID: PMC304864  PMID: 16593836

Abstract

Agrobacterium, Pseudomonas, and Rhizobium spp. have been made receptive to coliphage λ. To achieve this, recombinant (pTROY) plasmids carrying a constitutive Escherichia coli lamB gene encoding the λ receptor and expressed from an insertion sequence 3 (IS3) promoter were introduced into various bacteria. Because the wild-type λ receptor was not expressed in these bacteria, a procedure called the lamB gene tandem protocol was used to select lamB alleles that expressed the λ receptor. This gene tandem protocol may have general use in adapting genes with nonselectable traits to different organisms. Agrobacterium, Pseudomonas, and Rhizobium strains carrying pTROY41613, which encoded a gene tandem-selected λ receptor, could be quantitatively transduced with λ-packaged cosmids. With the ability to confer λ receptivity on organisms, phage λ now becomes a general DNA delivery agent.

Keywords: λ receptor protein, pTROY plasmids, cosmid transduction, λ DNA vectors

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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