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. 1965 Feb;89(2):462–469. doi: 10.1128/jb.89.2.462-469.1965

Physical Properties of Chick Interferon

Leo E Kreuz a,1, Allan H Levy a,2
PMCID: PMC305529  PMID: 14255715

Abstract

Kreuz, Leo E. (The Johns Hopkins University School of Medicine, Baltimore, Md.), and Allan H. Levy. Physical properties of chick interferon. J. Bacteriol. 89:462–469. 1965.—The sedimentation coefficient, diffusion coefficient, and molecular weight of chick-embryo interferon were determined by zone centrifugation and equilibrium sedimentation in cesium chloride density gradients, and by chromatography on Sephadex G-100 columns. Purified interferon is not available in quantities sufficient to permit direct analysis by chemical or physical means; its relative concentrations were determined, therefore, by bioassay. I125-human serum albumin was used as an internal reference in all experiments. The sedimentation coefficient of chick-embryo interferon is 2.2 to 2.3S; the diffusion coefficient is 9.5 × 10−7 cm2sec−1. A molecular weight of 26,000 was calculated from the sedimentation and diffusion coefficients, and a range of 25,000 to 34,000 daltons was obtained from equilibrium-sedimentation analyses.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ACKERS G. K. MOLECULAR EXCLUSION AND RESTRICTED DIFFUSION PROCESSES IN MOLECULAR-SIEVE CHROMATOGRAPHY. Biochemistry. 1964 May;3:723–730. doi: 10.1021/bi00893a021. [DOI] [PubMed] [Google Scholar]
  2. KREUZ L. E., LEVY A. H. DENSITY HOMOGENEITY AND ESTIMATED MOLECULAR WEIGHT OF INTERFERON. Nature. 1963 Nov 30;200:883–884. doi: 10.1038/200883a0. [DOI] [PubMed] [Google Scholar]
  3. LAMPSON G. P., TYTELL A. A., NEMES M. M., HILLEMAN M. R. Purification and characterization of chick embryo interferon. Proc Soc Exp Biol Med. 1963 Feb;112:468–478. doi: 10.3181/00379727-112-28080. [DOI] [PubMed] [Google Scholar]
  4. LINDENMANN J., GIFFORD G. E. Studies on vaccinia virus plaque formation and its inhibition by interferon. III. A simplified plaque inhibition assay of interferon. Virology. 1963 Mar;19:302–309. doi: 10.1016/0042-6822(63)90068-0. [DOI] [PubMed] [Google Scholar]
  5. MARTIN R. G., AMES B. N. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed] [Google Scholar]
  6. MERIGAN T. C. PURIFIED INTERFERONS: PHYSICAL PROPERTIES AND SPECIES SPECIFICITY. Science. 1964 Aug 21;145(3634):811–813. doi: 10.1126/science.145.3634.811-a. [DOI] [PubMed] [Google Scholar]
  7. Meselson M., Stahl F. W., Vinograd J. EQUILIBRIUM SEDIMENTATION OF MACROMOLECULES IN DENSITY GRADIENTS. Proc Natl Acad Sci U S A. 1957 Jul 15;43(7):581–588. doi: 10.1073/pnas.43.7.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. PEDERSEN K. O. Exclusion chromatography. Arch Biochem Biophys. 1962 Sep;Suppl 1:157–168. [PubMed] [Google Scholar]
  9. PHILLIPS A. W., WOOD R. D. SOME PHYSICAL PROPERTIES OF CHICK INTERFERON. Nature. 1964 Feb 22;201:819–820. doi: 10.1038/201819b0. [DOI] [PubMed] [Google Scholar]
  10. PORTERFIELD J. S., BURKE D. C., ALLISON A. C. An estimate of the molecular weight of interferon as measured by its rate of diffusion through agar. Virology. 1960 Oct;12:197–203. doi: 10.1016/0042-6822(60)90194-x. [DOI] [PubMed] [Google Scholar]
  11. ROTEM Z., CHARLWOOD P. A. Molecular weights of interferons from different animal species. Nature. 1963 Jun 15;198:1066–1068. doi: 10.1038/1981066a0. [DOI] [PubMed] [Google Scholar]
  12. SHULMAN S. The determination of sedimentation constants with the oilturbine and Spinco ultracentrifuges. Arch Biochem Biophys. 1953 May;44(1):230–240. doi: 10.1016/0003-9861(53)90028-9. [DOI] [PubMed] [Google Scholar]
  13. WAGNER R. R. Biological studies of interferon. I. Suppression of cellular infection with eastern equine encephalomyelitis virus. Virology. 1961 Mar;13:323–337. doi: 10.1016/0042-6822(61)90152-0. [DOI] [PubMed] [Google Scholar]
  14. WAGNER R. R., LEVY A. H. Interferon as a chemical intermediary in viral interference. Ann N Y Acad Sci. 1960 Nov 21;88:1308–1318. doi: 10.1111/j.1749-6632.1960.tb20122.x. [DOI] [PubMed] [Google Scholar]
  15. WAGNER R. R. THE INTERFERONS: CELLULAR INHIBITORS OF VIRAL INFECTION. Annu Rev Microbiol. 1963;17:285–296. doi: 10.1146/annurev.mi.17.100163.001441. [DOI] [PubMed] [Google Scholar]

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