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. 1965 Feb;89(2):462–469. doi: 10.1128/jb.89.2.462-469.1965

Physical Properties of Chick Interferon

Leo E Kreuz a,1, Allan H Levy a,2
PMCID: PMC305529  PMID: 14255715

Abstract

Kreuz, Leo E. (The Johns Hopkins University School of Medicine, Baltimore, Md.), and Allan H. Levy. Physical properties of chick interferon. J. Bacteriol. 89:462–469. 1965.—The sedimentation coefficient, diffusion coefficient, and molecular weight of chick-embryo interferon were determined by zone centrifugation and equilibrium sedimentation in cesium chloride density gradients, and by chromatography on Sephadex G-100 columns. Purified interferon is not available in quantities sufficient to permit direct analysis by chemical or physical means; its relative concentrations were determined, therefore, by bioassay. I125-human serum albumin was used as an internal reference in all experiments. The sedimentation coefficient of chick-embryo interferon is 2.2 to 2.3S; the diffusion coefficient is 9.5 × 10−7 cm2sec−1. A molecular weight of 26,000 was calculated from the sedimentation and diffusion coefficients, and a range of 25,000 to 34,000 daltons was obtained from equilibrium-sedimentation analyses.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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