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. 1987 Jun 25;15(12):4761–4770. doi: 10.1093/nar/15.12.4761

Novel non-isotopic in situ hybridization technique detects small (1 Kb) unique sequences in routinely G-banded human chromosomes: fine mapping of N-myc and beta-NGF genes.

J A Garson, J A van den Berghe, J T Kemshead
PMCID: PMC305916  PMID: 3299258

Abstract

A novel in situ hybridization technique is described. This non-radioactive technique combines, for the first time, the high spacial resolution and rapid signal development of the non-isotopic approach with the previously unrivalled sensitivity of autoradiography. The procedure, which employs biotin labelled DNA probes and a streptavidin-alkaline phosphatase based detection system, is compatible with pre G-banding and can be performed on archival material. Unique sequences as small as 1 Kb are detectable. Using this technique, we have mapped the N-myc oncogene and the gene for beta-Nerve Growth Factor to 2p24 and 1p13 respectively.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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