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. 1987 Jul 10;15(13):5017–5030. doi: 10.1093/nar/15.13.5017

The yeast DNA polymerase I transcript is regulated in both the mitotic cell cycle and in meiosis and is also induced after DNA damage.

L H Johnston, J H White, A L Johnson, G Lucchini, P Plevani
PMCID: PMC305944  PMID: 3299263

Abstract

Using mitotic cultures synchronised by a feed-starve protocol or by elutriation, we have shown that the yeast DNA polymerase I gene is periodically expressed with its transcript increasing at least 100-fold in late G1 with a peak around the G1/S phase boundary. This is precisely the same interval of the cell cycle in which three other yeast DNA synthesis genes, CDC8, CDC9 and CDC21, have been found to be periodically expressed (White et al 1987. Expl. Cell. Res., in press). The polymerase I transcript is also regulated in meiosis, showing an overall fluctuation in level of some 20-fold, with a peak at about mid-S phase. In addition, following irradiation with 50J/m2 ultraviolet light, there was a 20-fold increase in the transcript, starting after 30 minutes and reaching a peak two hours later. These results indicate that DNA polymerase I is subject to a complex control and imply that it has a role in both DNA synthesis and DNA repair.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aves S. J., Durkacz B. W., Carr A., Nurse P. Cloning, sequencing and transcriptional control of the Schizosaccharomyces pombe cdc10 'start' gene. EMBO J. 1985 Feb;4(2):457–463. doi: 10.1002/j.1460-2075.1985.tb03651.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Badaracco G., Capucci L., Plevani P., Chang L. M. Polypeptide structure of DNA polymerase I from Saccharomyces cerevisiae. J Biol Chem. 1983 Sep 10;258(17):10720–10726. [PubMed] [Google Scholar]
  3. Chang L. M. DNA polymerases from bakers' yeast. J Biol Chem. 1977 Mar 25;252(6):1873–1880. [PubMed] [Google Scholar]
  4. Eckstein H., Paduch V., Hilz H. Synchronized yeast cells. 3. DNA synthesis and DNA polymerase after inhibition of cell division by x-rays. Eur J Biochem. 1967 Dec;3(2):224–231. doi: 10.1111/j.1432-1033.1967.tb19520.x. [DOI] [PubMed] [Google Scholar]
  5. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  6. Golombek J., Wolf W., Wintersberger E. DNA synthesis and DNA-polymerase activity in synchronized yeast cells. Mol Gen Genet. 1974;132(2):137–145. doi: 10.1007/BF00272179. [DOI] [PubMed] [Google Scholar]
  7. Hereford L. M., Osley M. A., Ludwig T. R., 2nd, McLaughlin C. S. Cell-cycle regulation of yeast histone mRNA. Cell. 1981 May;24(2):367–375. doi: 10.1016/0092-8674(81)90326-3. [DOI] [PubMed] [Google Scholar]
  8. Johnson A. L., Barker D. G., Johnston L. H. Induction of yeast DNA ligase genes in exponential and stationary phase cultures in response to DNA damaging agents. Curr Genet. 1986;11(2):107–112. doi: 10.1007/BF00378201. [DOI] [PubMed] [Google Scholar]
  9. Johnson L. M., Snyder M., Chang L. M., Davis R. W., Campbell J. L. Isolation of the gene encoding yeast DNA polymerase I. Cell. 1985 Nov;43(1):369–377. doi: 10.1016/0092-8674(85)90042-x. [DOI] [PubMed] [Google Scholar]
  10. Johnston L. H., Johnson A. L., Barker D. G. The expression in meiosis of genes which are transcribed periodically in the mitotic cell cycle of budding yeast. Exp Cell Res. 1986 Aug;165(2):541–549. doi: 10.1016/0014-4827(86)90606-3. [DOI] [PubMed] [Google Scholar]
  11. Johnston L. H., Thomas A. P. A further two mutants defective in initiation of the S phase in the yeast Saccharomyces cerevisiae. Mol Gen Genet. 1982;186(3):445–448. doi: 10.1007/BF00729467. [DOI] [PubMed] [Google Scholar]
  12. Johnston L. H., Thomas A. P. The isolation of new DNA synthesis mutants in the yeast Saccharomyces cerevisiae. Mol Gen Genet. 1982;186(3):439–444. doi: 10.1007/BF00729466. [DOI] [PubMed] [Google Scholar]
  13. Kupiec M., Simchen G. Regulation of the RAD6 gene of Saccharomyces cerevisiae in the mitotic cell cycle and in meiosis. Mol Gen Genet. 1986 Jun;203(3):538–543. doi: 10.1007/BF00422083. [DOI] [PubMed] [Google Scholar]
  14. Lowdon M., Vitols E. Ribonucleotide reductase activity during the cell cycle of Saccharomyces cerevisiae. Arch Biochem Biophys. 1973 Sep;158(1):177–184. doi: 10.1016/0003-9861(73)90611-5. [DOI] [PubMed] [Google Scholar]
  15. Lucchini G., Brandazza A., Badaracco G., Bianchi M., Plevani P. Identification of the yeast DNA polymerase I gene with antibody probes. Curr Genet. 1985;10(4):245–252. doi: 10.1007/BF00365620. [DOI] [PubMed] [Google Scholar]
  16. Maga J. A., McClanahan T. A., McEntee K. Transcriptional regulation of DNA damage responsive (DDR) genes in different rad mutant strains of Saccharomyces cerevisiae. Mol Gen Genet. 1986 Nov;205(2):276–284. doi: 10.1007/BF00430439. [DOI] [PubMed] [Google Scholar]
  17. Peterson T. A., Prakash L., Prakash S., Osley M. A., Reed S. I. Regulation of CDC9, the Saccharomyces cerevisiae gene that encodes DNA ligase. Mol Cell Biol. 1985 Jan;5(1):226–235. doi: 10.1128/mcb.5.1.226. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Plevani P., Badaracco G., Augl C., Chang L. M. DNA polymerase I and DNA primase complex in yeast. J Biol Chem. 1984 Jun 25;259(12):7532–7539. [PubMed] [Google Scholar]
  19. Robinson G. W., Nicolet C. M., Kalainov D., Friedberg E. C. A yeast excision-repair gene is inducible by DNA damaging agents. Proc Natl Acad Sci U S A. 1986 Mar;83(6):1842–1846. doi: 10.1073/pnas.83.6.1842. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ruby S. W., Szostak J. W. Specific Saccharomyces cerevisiae genes are expressed in response to DNA-damaging agents. Mol Cell Biol. 1985 Jan;5(1):75–84. doi: 10.1128/mcb.5.1.75. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Simchen G. Are mitotic functions required in meiosis? Genetics. 1974 Apr;76(4):745–753. doi: 10.1093/genetics/76.4.745. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Storms R. K., Ord R. W., Greenwood M. T., Mirdamadi B., Chu F. K., Belfort M. Cell cycle-dependent expression of thymidylate synthase in Saccharomyces cerevisiae. Mol Cell Biol. 1984 Dec;4(12):2858–2864. doi: 10.1128/mcb.4.12.2858. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. White J. H., Barker D. G., Nurse P., Johnston L. H. Periodic transcription as a means of regulating gene expression during the cell cycle: contrasting modes of expression of DNA ligase genes in budding and fission yeast. EMBO J. 1986 Jul;5(7):1705–1709. doi: 10.1002/j.1460-2075.1986.tb04414.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Williamson D. H., Johnston L. H., Fennell D. J., Simchen G. The timing of the S phase and other nuclear events in yeast meiosis. Exp Cell Res. 1983 Apr 15;145(1):209–217. doi: 10.1016/s0014-4827(83)80022-6. [DOI] [PubMed] [Google Scholar]

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