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. 1987 Oct 12;15(19):7865–7876. doi: 10.1093/nar/15.19.7865

Resolution of DNA molecules greater than 5 megabases by contour-clamped homogeneous electric fields.

D Vollrath 1, R W Davis 1
PMCID: PMC306313  PMID: 2959907

Abstract

Excellent resolution of chromosomal DNA molecules from Saccharomyces cerevisiae, Candida albicans and Schizosaccharomyces pombe has been obtained using alternating contour-clamped homogeneous electric field (CHEF) gel electrophoresis. The largest of these molecules is greater than 5 Mb in size and is resolved after 130 hours in a 0.6% agarose gel at a field strength of 1.3 V/cm and a switching interval of 1 hour. Separation of concatamers of phage lambda DNA reveals four regions of resolution in alternating CHEF gel electrophoresis. There are two regions of good resolution in which mobility approximates a linear function of molecular weight. These are separated by a region of lower resolution and bounded at high molecular weights by a region of little or no resolution. The four regions are of practical and possibly theoretical importance.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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