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. 1987 Nov 11;15(21):8643–8659. doi: 10.1093/nar/15.21.8643

Sequence-targeted chemical modifications of nucleic acids by complementary oligonucleotides covalently linked to porphyrins.

Trung Le Doan 1, L Perrouault 1, M Chassignol 1, T T Nguyen 1, C Hélène 1
PMCID: PMC306396  PMID: 3684568

Abstract

Oligo-heptathymidylates covalently linked to porphyrins bind to complementary sequences and can induce local damages on the target molecule. In dark reactions, iron porphyrin derivatives exhibited various chemical reactivities resulting in base oxidation, crosslinking and chain scission reactions. Reactions induced by reductants, such as ascorbic acid, dithiothreitol or mercapto-propionic acid, led to very localised reactions. A single base was the target for more than 50% of the damages. Oxidising agents such as H2O2 and its alkyl derivatives induced reactions that extended to a wider range of altered bases. The specificity of the chemical modifications observed in these systems is discussed from a mechanistic point of view.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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