Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1987 Nov 11;15(21):8957–8962. doi: 10.1093/nar/15.21.8957

Linkage of TNF genes to the HLA-B locus.

H Inoko 1, J Trowsdale 1
PMCID: PMC306415  PMID: 3479750

Abstract

Pulsed field gel electrophoresis was used to determine the location of the tumour necrosis factor (TNF) alpha and beta genes. They were shown to be linked to the HLA-B locus; analogous to their location in mouse, between the complement (class III) region and H-2D. However, the distance between the TNF genes and the class I region was much greater in man, namely about 260 kb, compared to 70 kb in the mouse. This finding may have implications for some HLA associated diseases.

Full text

PDF
8959

Images in this article

8959Image
on p.8959

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aggarwal B. B., Kohr W. J., Hass P. E., Moffat B., Spencer S. A., Henzel W. J., Bringman T. S., Nedwin G. E., Goeddel D. V., Harkins R. N. Human tumor necrosis factor. Production, purification, and characterization. J Biol Chem. 1985 Feb 25;260(4):2345–2354. [PubMed] [Google Scholar]
  2. Benton W. D., Davis R. W. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977 Apr 8;196(4286):180–182. doi: 10.1126/science.322279. [DOI] [PubMed] [Google Scholar]
  3. Bernards A., Kooter J. M., Michels P. A., Moberts R. M., Borst P. Pulsed field gradient electrophoresis of DNA digested in agarose allows the sizing of the large duplication unit of a surface antigen gene in trypanosomes. Gene. 1986;42(3):313–322. doi: 10.1016/0378-1119(86)90235-0. [DOI] [PubMed] [Google Scholar]
  4. Beutler B., Cerami A. Cachectin and tumour necrosis factor as two sides of the same biological coin. Nature. 1986 Apr 17;320(6063):584–588. doi: 10.1038/320584a0. [DOI] [PubMed] [Google Scholar]
  5. Carle G. F., Olson M. V. Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis. Nucleic Acids Res. 1984 Jul 25;12(14):5647–5664. doi: 10.1093/nar/12.14.5647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Carswell E. A., Old L. J., Kassel R. L., Green S., Fiore N., Williamson B. An endotoxin-induced serum factor that causes necrosis of tumors. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3666–3670. doi: 10.1073/pnas.72.9.3666. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  8. Geczy A. F., Alexander K., Bashir H. V., Edmonds J. P., Upfold L., Sullivan J. HLA-B27, Klebsiella and ankylosing spondylitis: biological and chemical studies. Immunol Rev. 1983;70:23–50. doi: 10.1111/j.1600-065x.1983.tb00708.x. [DOI] [PubMed] [Google Scholar]
  9. Inoko H., Ando A., Kimura M., Tsuji K. Isolation and characterization of the cDNA clone and genomic clones of a new HLA class II antigen heavy chain, DO alpha. J Immunol. 1985 Sep;135(3):2156–2159. [PubMed] [Google Scholar]
  10. Koller B. H., Sidwell B., DeMars R., Orr H. T. Isolation of HLA locus-specific DNA probes from the 3'-untranslated region. Proc Natl Acad Sci U S A. 1984 Aug;81(16):5175–5178. doi: 10.1073/pnas.81.16.5175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Müller U., Jongeneel C. V., Nedospasov S. A., Lindahl K. F., Steinmetz M. Tumour necrosis factor and lymphotoxin genes map close to H-2D in the mouse major histocompatibility complex. Nature. 1987 Jan 15;325(6101):265–267. doi: 10.1038/325265a0. [DOI] [PubMed] [Google Scholar]
  12. Müller U., Stephan D., Philippsen P., Steinmetz M. Orientation and molecular map position of the complement genes in the mouse MHC. EMBO J. 1987 Feb;6(2):369–373. doi: 10.1002/j.1460-2075.1987.tb04764.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nedospasov S. A., Hirt B., Shakhov A. N., Dobrynin V. N., Kawashima E., Accolla R. S., Jongeneel C. V. The genes for tumor necrosis factor (TNF-alpha) and lymphotoxin (TNF-beta) are tandemly arranged on chromosome 17 of the mouse. Nucleic Acids Res. 1986 Oct 10;14(19):7713–7725. doi: 10.1093/nar/14.19.7713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nedospasov S. A., Shakhov A. N., Turetskaia R. L., Mett V. A., Georgiev G. P. Molekuliarnoe klonirovanie genov cheloveka, kodiruiushchikh faktory nekroza opukholei: tandemnoe raspolozhenie al'fa- i beta-genov v korotkom segmente (6 tys. par nukleotidov) genoma cheloveka. Dokl Akad Nauk SSSR. 1985;285(6):1487–1490. [PubMed] [Google Scholar]
  15. Nedwin G. E., Naylor S. L., Sakaguchi A. Y., Smith D., Jarrett-Nedwin J., Pennica D., Goeddel D. V., Gray P. W. Human lymphotoxin and tumor necrosis factor genes: structure, homology and chromosomal localization. Nucleic Acids Res. 1985 Sep 11;13(17):6361–6373. doi: 10.1093/nar/13.17.6361. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Old L. J. Tumor necrosis factor (TNF). Science. 1985 Nov 8;230(4726):630–632. doi: 10.1126/science.2413547. [DOI] [PubMed] [Google Scholar]
  17. Ozawa A., Ohkido M., Tsuji K. Some recent advances in HLA and skin diseases. J Am Acad Dermatol. 1981 Feb;4(2):205–230. doi: 10.1016/s0190-9622(81)70024-0. [DOI] [PubMed] [Google Scholar]
  18. Schwartz D. C., Cantor C. R. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell. 1984 May;37(1):67–75. doi: 10.1016/0092-8674(84)90301-5. [DOI] [PubMed] [Google Scholar]
  19. Shirai T., Yamaguchi H., Ito H., Todd C. W., Wallace R. B. Cloning and expression in Escherichia coli of the gene for human tumour necrosis factor. 1985 Feb 28-Mar 6Nature. 313(6005):803–806. doi: 10.1038/313803a0. [DOI] [PubMed] [Google Scholar]
  20. Sood A. K., Pereira D., Weissman S. M. Isolation and partial nucleotide sequence of a cDNA clone for human histocompatibility antigen HLA-B by use of an oligodeoxynucleotide primer. Proc Natl Acad Sci U S A. 1981 Jan;78(1):616–620. doi: 10.1073/pnas.78.1.616. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Spies T., Morton C. C., Nedospasov S. A., Fiers W., Pious D., Strominger J. L. Genes for the tumor necrosis factors alpha and beta are linked to the human major histocompatibility complex. Proc Natl Acad Sci U S A. 1986 Nov;83(22):8699–8702. doi: 10.1073/pnas.83.22.8699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Strachan T., Dodge A. B., Smillie D., Dyer P. A., Sodoyer R., Jordan B. R., Harris R. An HLA-C-specific DNA probe. Immunogenetics. 1986;23(2):115–120. doi: 10.1007/BF00377971. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES