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. 1995 Jun 25;23(12):2300–2304. doi: 10.1093/nar/23.12.2300

Phylogenetic analysis of Leishmania RNA virus and Leishmania suggests ancient virus-parasite association.

G Widmer 1, S Dooley 1
PMCID: PMC307021  PMID: 7610059

Abstract

Some strains of the protozoan parasite Leishmania belonging to the new world species guyanensis and braziliensis are infected with persistent, single-segmented, non-enveloped dsRNA viruses termed LRV1. A single old world strain classified as L. major was recently found to harbor a similar virus, designated LRV2-1. The genomic nucleotide sequences of two LRV1 types (1-1 and 1-4) isolated from two L. guyanensis strains have been determined and found to be highly conserved. In contrast, LRV1-specific cDNA probes derived from the conserved genomic 5' region failed to recognize LRV2 RNA on Northern blots, suggesting a greater degree of divergence between LRV1 and LRV2 than among LRV1 types. This observation suggests a long-term association and coevolution of LRV within each parasite strain. We tested this concept by comparing nucleotide sequences of seven LRV types and PCR fingerprints of the parasite strains from which these viruses were derived. In support of the idea of virus-parasite co-evolution, we find that genetic distances between LRV types mirror the heterogeneity between parasite fingerprints and are clustered according to the geographical origin of the strains. In agreement with the postulated common origin of persistent dsRNA viruses of protozoa and fungi, we conclude that the infection of Leishmania with LRV pre-dates the divergence of Leishmania into different lineages.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beverley S. M., Ismach R. B., Pratt D. M. Evolution of the genus Leishmania as revealed by comparisons of nuclear DNA restriction fragment patterns. Proc Natl Acad Sci U S A. 1987 Jan;84(2):484–488. doi: 10.1073/pnas.84.2.484. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bruenn J. A. A closely related group of RNA-dependent RNA polymerases from double-stranded RNA viruses. Nucleic Acids Res. 1993 Dec 11;21(24):5667–5669. doi: 10.1093/nar/21.24.5667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cadd T. L., Keenan M. C., Patterson J. L. Detection of Leishmania RNA virus 1 proteins. J Virol. 1993 Sep;67(9):5647–5650. doi: 10.1128/jvi.67.9.5647-5650.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cadd T. L., Patterson J. L. Synthesis of viruslike particles by expression of the putative capsid protein of Leishmania RNA virus in a recombinant baculovirus expression system. J Virol. 1994 Jan;68(1):358–365. doi: 10.1128/jvi.68.1.358-365.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Coderre J. A., Beverley S. M., Schimke R. T., Santi D. V. Overproduction of a bifunctional thymidylate synthetase-dihydrofolate reductase and DNA amplification in methotrexate-resistant Leishmania tropica. Proc Natl Acad Sci U S A. 1983 Apr;80(8):2132–2136. doi: 10.1073/pnas.80.8.2132. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Guilbride L., Myler P. J., Stuart K. Distribution and sequence divergence of LRV1 viruses among different Leishmania species. Mol Biochem Parasitol. 1992 Aug;54(1):101–104. doi: 10.1016/0166-6851(92)90099-6. [DOI] [PubMed] [Google Scholar]
  7. Holland J., Spindler K., Horodyski F., Grabau E., Nichol S., VandePol S. Rapid evolution of RNA genomes. Science. 1982 Mar 26;215(4540):1577–1585. doi: 10.1126/science.7041255. [DOI] [PubMed] [Google Scholar]
  8. Kreutzer R. D., Souraty N., Semko M. E. Biochemical identities and differences among Leishmania species and subspecies. Am J Trop Med Hyg. 1987 Jan;36(1):22–32. doi: 10.4269/ajtmh.1987.36.22. [DOI] [PubMed] [Google Scholar]
  9. Panton L. J., Tesh R. B., Nadeau K. C., Beverley S. M. A test for genetic exchange in mixed infections of Leishmania major in the sand fly Phlebotomus papatasi. J Protozool. 1991 May-Jun;38(3):224–228. doi: 10.1111/j.1550-7408.1991.tb04433.x. [DOI] [PubMed] [Google Scholar]
  10. Scheffter S., Widmer G., Patterson J. L. Complete sequence of Leishmania RNA virus 1-4 and identification of conserved sequences. Virology. 1994 Mar;199(2):479–483. doi: 10.1006/viro.1994.1149. [DOI] [PubMed] [Google Scholar]
  11. Stuart K. D., Weeks R., Guilbride L., Myler P. J. Molecular organization of Leishmania RNA virus 1. Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8596–8600. doi: 10.1073/pnas.89.18.8596. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Tarr P. I., Aline R. F., Jr, Smiley B. L., Scholler J., Keithly J., Stuart K. LR1: a candidate RNA virus of Leishmania. Proc Natl Acad Sci U S A. 1988 Dec;85(24):9572–9575. doi: 10.1073/pnas.85.24.9572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Tibayrenc M., Kjellberg F., Arnaud J., Oury B., Brenière S. F., Dardé M. L., Ayala F. J. Are eukaryotic microorganisms clonal or sexual? A population genetics vantage. Proc Natl Acad Sci U S A. 1991 Jun 15;88(12):5129–5133. doi: 10.1073/pnas.88.12.5129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wang A., Wang C. C., Alderete J. F. Trichomonas vaginalis phenotypic variation occurs only among trichomonads infected with the double-stranded RNA virus. J Exp Med. 1987 Jul 1;166(1):142–150. doi: 10.1084/jem.166.1.142. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Welsh J., McClelland M. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 1990 Dec 25;18(24):7213–7218. doi: 10.1093/nar/18.24.7213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Widmer G., Comeau A. M., Furlong D. B., Wirth D. F., Patterson J. L. Characterization of a RNA virus from the parasite Leishmania. Proc Natl Acad Sci U S A. 1989 Aug;86(15):5979–5982. doi: 10.1073/pnas.86.15.5979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Widmer G., Patterson J. L. Genomic structure and RNA polymerase activity in Leishmania virus. J Virol. 1991 Aug;65(8):4211–4215. doi: 10.1128/jvi.65.8.4211-4215.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. van Eys G. J., Schoone G. J., Kroon N. C., Ebeling S. B. Sequence analysis of small subunit ribosomal RNA genes and its use for detection and identification of Leishmania parasites. Mol Biochem Parasitol. 1992 Mar;51(1):133–142. doi: 10.1016/0166-6851(92)90208-2. [DOI] [PubMed] [Google Scholar]

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