Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1995 Aug 11;23(15):3071–3072. doi: 10.1093/nar/23.15.3071

A rapid RT-PCR method for detection of intact RNA in formalin-fixed paraffin-embedded tissues.

Y H Jiang 1, L A Davidson 1, J R Lupton 1, R S Chapkin 1
PMCID: PMC307153  PMID: 7544892

Abstract

Full text

PDF
3072

Images in this article

3072Image
on p.3072
3072Image
on p.3072

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  2. Davidson L. A., Jiang Y. H., Derr J. N., Aukema H. M., Lupton J. R., Chapkin R. S. Protein kinase C isoforms in human and rat colonic mucosa. Arch Biochem Biophys. 1994 Aug 1;312(2):547–553. doi: 10.1006/abbi.1994.1344. [DOI] [PubMed] [Google Scholar]
  3. Ferre F., Garduno F. Preparation of crude cell extract suitable for amplification of RNA by the polymerase chain reaction. Nucleic Acids Res. 1989 Mar 11;17(5):2141–2141. doi: 10.1093/nar/17.5.2141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Mies C. Molecular pathology of paraffin-embedded tissue. Current clinical applications. Diagn Mol Pathol. 1992 Sep;1(3):206–211. [PubMed] [Google Scholar]
  5. Nuovo G. J., Gallery F., MacConnell P., Braun A. In situ detection of polymerase chain reaction-amplified HIV-1 nucleic acids and tumor necrosis factor-alpha RNA in the central nervous system. Am J Pathol. 1994 Apr;144(4):659–666. [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES