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. 1995 Oct 11;23(19):3816–3821. doi: 10.1093/nar/23.19.3816

Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase.

Y Kanegae 1, G Lee 1, Y Sato 1, M Tanaka 1, M Nakai 1, T Sakaki 1, S Sugano 1, I Saito 1
PMCID: PMC307296  PMID: 7479022

Abstract

A recombinant adenovirus (Ad) expressing Cre recombinase derived from bacteriophage P1 was constructed. To assay the Cre activity in mammalian cells, another recombinant Ad bearing an on/off-switching reporter unit, where a LacZ-expression unit can be activated by the Cre-mediated excisional deletion of an interposed stuffer DNA, was also constructed. Co-infection experiments together with the Cre-expressing and the reporter recombinant Ads showed that the Cre-mediated switching of gene expression was detected in nearly 100% of cultured CV1, HeLa and Jurkat cells. These results suggest that the recombinant Ad efficiently expressed functional Cre and offers a basis for establishing a powerful on/off switching strategy of gene expression in cultured mammalian cells and presumably in transgenic animals. The method is also applicable to construction of recombinant Ad bearing a gene the expression of which is deleterious to propagation of recombinant Ad.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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