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. 1994 Jan 11;22(1):20–24. doi: 10.1093/nar/22.1.20

Oligonucleotide duplexes containing 2'-amino-2'-deoxycytidines: thermal stability and chemical reactivity.

H Aurup 1, T Tuschl 1, F Benseler 1, J Ludwig 1, F Eckstein 1
PMCID: PMC307740  PMID: 8127651

Abstract

Thermal stabilities of oligonucleotides containing 2'-amino-2'-deoxycytidines were determined and compared to those of the unmodified oligonucleotides. The presence of the 2'-aminonucleoside destabilized duplexes in a RNA as well as a DNA context at pH 7 as well as at pH 5. The pKa of the 2'-amino group was determined by 13C-NMR spectroscopy to be 6.2. The reactivity of an oligonucleotide containing a 2'-aminonucleoside was exploited for the incorporation of rhodamine by its isothiocyanate derivative.

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Selected References

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