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. 1994 May 25;22(10):1861–1865. doi: 10.1093/nar/22.10.1861

Highly efficient DNA synthesis in isolated mitochondria from rat liver.

J A Enríquez 1, J Ramos 1, A Pérez-Martos 1, M J López-Pérez 1, J Montoya 1
PMCID: PMC308085  PMID: 8208611

Abstract

We have developed a highly efficient DNA-synthesizing system with isolated intact rat liver mitochondria. The ATP requirements for this in organello DNA synthesis are provided by endogenous synthesis in the presence of exogenous ADP and an oxidizable substrate. In this system, mitochondrial DNA synthesis strikingly proceeds at a constant rate for about 5 h at 37 degrees C. Gel electrophoresis, hybridization and restriction enzyme analyses show that intact mitochondria synthesize nucleic acids with a size of 16.5 kb, that correspond to mitochondrial DNA, and that both DNA strands are replicated. This in organello DNA synthesis requires the supply of dNTPs and decreases at high ADP concentration in the incubation medium.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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