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. 1993 Mar 11;21(5):1239–1244. doi: 10.1093/nar/21.5.1239

Exoquence DNA sequencing.

C Li 1, P W Tucker 1
PMCID: PMC309288  PMID: 8464708

Abstract

We have developed a strategy for DNA sequencing based on exonuclease III digestion followed by double strand specific endonuclease digestion and direct dideoxynucleotide sequencing reaction. This strategy eliminates the need for subcloning, oligonucleotide primers, and prior knowledge of the DNA to be sequenced. All template and primer duplexes needed for sequencing a complete insert can be prepared in one day from uncharacterized starting DNA. Sequence information can be obtained from different regions of the DNA simultaneously. The method uses double-stranded DNA to generate single-stranded template and primer, and thus produces high quality sequence results. Commercially available dideoxy-sequencing kits are well suited for this method. The strategy should be applicable for both automatic and routine laboratory DNA sequencing.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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