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. 1993 Apr 25;21(8):1873–1879. doi: 10.1093/nar/21.8.1873

Transactivation of mouse insulin-like growth factor II (IGF-II) gene promoters by the AP-1 complex.

A Caricasole 1, A Ward 1
PMCID: PMC309427  PMID: 8493103

Abstract

The mouse insulin-like growth factor II gene (Igf2) is transcribed from three promoters (P1, P2 and P3), and is expressed in a tissue-specific and developmentally regulated fashion; however, little information is available on the transcription factors controlling Igf2 expression. The AP-1 complex is a transcription factor involved in the regulation of a variety of genes, including those encoding certain growth factors. We show that Igf2 P3 is transactivated by AP-1 in a transient expression assay, and that this effect is mediated through two non-consensus AP-1 binding sites characterised by DNA-protein interaction studies. Mutational analysis indicates these sites are required for AP-1 responsiveness and full promoter activity.

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