Skip to main content
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1993 Jun 25;21(12):2959–2960. doi: 10.1093/nar/21.12.2959

An alternative hot start technique for PCR in small volumes using beads of wax-embedded reaction components dried in trehalose.

S Kaijalainen 1, P J Karhunen 1, K Lalu 1, K Lindström 1
PMCID: PMC309708  PMID: 8332517

Abstract

Full text

PDF
2960

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen R. C., Graves G., Budowle B. Polymerase chain reaction amplification products separated on rehydratable polyacrylamide gels and stained with silver. Biotechniques. 1989 Jul-Aug;7(7):736–744. [PubMed] [Google Scholar]
  2. Colaço C., Sen S., Thangavelu M., Pinder S., Roser B. Extraordinary stability of enzymes dried in trehalose: simplified molecular biology. Biotechnology (N Y) 1992 Sep;10(9):1007–1011. doi: 10.1038/nbt0992-1007. [DOI] [PubMed] [Google Scholar]
  3. Edwards A., Civitello A., Hammond H. A., Caskey C. T. DNA typing and genetic mapping with trimeric and tetrameric tandem repeats. Am J Hum Genet. 1991 Oct;49(4):746–756. [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES