Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1993 Jul 11;21(14):3337–3338. doi: 10.1093/nar/21.14.3337

Simplified high throughput protocol for northern hybridization.

H Yang 1, J McLeese 1, M Weisbart 1, J L Dionne 1, I Lemaire 1, R A Aubin 1
PMCID: PMC309787  PMID: 8341618

Abstract

Full text

PDF
3337

Images in this article

3338Image
on p.3338
3338Image
on p.3338
3338Image
on p.3338

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Becker S., Devlin R. B., Haskill J. S. Differential production of tumor necrosis factor, macrophage colony stimulating factor, and interleukin 1 by human alveolar macrophages. J Leukoc Biol. 1989 Apr;45(4):353–361. [PubMed] [Google Scholar]
  2. Beutler B., Krochin N., Milsark I. W., Luedke C., Cerami A. Control of cachectin (tumor necrosis factor) synthesis: mechanisms of endotoxin resistance. Science. 1986 May 23;232(4753):977–980. doi: 10.1126/science.3754653. [DOI] [PubMed] [Google Scholar]
  3. Britten R. J., Graham D. E., Neufeld B. R. Analysis of repeating DNA sequences by reassociation. Methods Enzymol. 1974;29:363–418. doi: 10.1016/0076-6879(74)29033-5. [DOI] [PubMed] [Google Scholar]
  4. Budowle B., Baechtel F. S. Modifications to improve the effectiveness of restriction fragment length polymorphism typing. Appl Theor Electrophor. 1990;1(4):181–187. [PubMed] [Google Scholar]
  5. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  6. Church G. M., Gilbert W. Genomic sequencing. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991–1995. doi: 10.1073/pnas.81.7.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  8. Gosset P., Lassalle P., Vanhée D., Wallaert B., Aerts C., Voisin C., Tonnel A. B. Production of tumor necrosis factor-alpha and interleukin-6 by human alveolar macrophages exposed in vitro to coal mine dust. Am J Respir Cell Mol Biol. 1991 Nov;5(5):431–436. doi: 10.1165/ajrcmb/5.5.431. [DOI] [PubMed] [Google Scholar]
  9. Lee F. J., Moss J., Lin L. W. A simplified procedure for hybridization of RNA blots. Biotechniques. 1992 Dec;13(6):844–846. [PubMed] [Google Scholar]
  10. Lemaire I., Beaudoin H., Massé S., Grondin C. Alveolar macrophage stimulation of lung fibroblast growth in asbestos-induced pulmonary fibrosis. Am J Pathol. 1986 Feb;122(2):205–211. [PMC free article] [PubMed] [Google Scholar]
  11. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  12. Ulich T. R., Watson L. R., Yin S. M., Guo K. Z., Wang P., Thang H., del Castillo J. The intratracheal administration of endotoxin and cytokines. I. Characterization of LPS-induced IL-1 and TNF mRNA expression and the LPS-, IL-1-, and TNF-induced inflammatory infiltrate. Am J Pathol. 1991 Jun;138(6):1485–1496. [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES