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. 1986 Jul 25;14(14):5919–5931. doi: 10.1093/nar/14.14.5919

The influence of ionic strength on the binding of a water soluble porphyrin to nucleic acids.

R F Pasternack, P Garrity, B Ehrlich, C B Davis, E J Gibbs, G Orloff, A Giartosio, C Turano
PMCID: PMC311600  PMID: 3737417

Abstract

The ionic strength dependences of the binding of tetrakis (4-N-methylpyridyl)porphine (H2TMpyP) to poly(dG-dC) and calf thymus DNA have been determined. For the former system the results are typical of other intercalators, i.e., a plot of log K vs log [Na+] is linear albeit with a slope which suggests that the "effective charge" of the porphyrin is closer to two than the formal charge of +4. For calf thymus DNA, the binding profile is not completely compatible with the predictions of condensation theory. Whereas the avidity of binding does decrease with increasing [Na+] as predicted, of greater interest is the relocation of the porphyrin from GC-rich regions to AT-rich regions as the ionic strength increases.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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