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. 1986 Aug 26;14(16):6525–6540. doi: 10.1093/nar/14.16.6525

Application of new catalytic phosphate protecting groups for the highly efficient phosphotriester oligonucleotide synthesis.

V A Efimov, A A Buryakova, I Y Dubey, N N Polushin, O G Chakhmakhcheva, Ovchinnikov YuA
PMCID: PMC311661  PMID: 3748819

Abstract

An effective procedure for the synthesis of oligonucleotides by the phosphotriester method has been developed. The procedure is based on the use of phosphate protecting groups enabling O-nucleophilic intramolecular catalysis in the reaction of internucleotide bond formation under the action of arylsulfonyl chlorides and their derivatives. Using this new procedure, the time needed to perform one elongation step on polymer support is 7-8 min. The effectiveness of the methodology has been demonstrated in the synthesis of many oligodeoxyribonucleotides of different length with high yields.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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