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. 1986 Sep 11;14(17):7031–7045. doi: 10.1093/nar/14.17.7031

Restriction endonucleases from Herpetosiphon giganteus: an example of the evolution of DNA recognition specificity?

P R Whitehead, D Jacobs, N L Brown
PMCID: PMC311715  PMID: 3020503

Abstract

We describe the partial purification and characterisation of five Type II restriction endonucleases from two strains of Herpetosiphon giganteus. One of the activities, HgiJII, was the first enzyme found that cleaves DNA at the family of related sequences 5'-G-R-G-C-Y/C-3'. This enzyme may be related to the enzyme HgiAI from a different strain of the same species, and which cleaves at the sites 5'-G-W-G-C-W/C-3'. We have shown that DNAs from the strains producing HgiAI and HgiJII are resistant to both of these restriction endonucleases. The remaining four enzymes described here share recognition and cleavage specificities with other restriction endonucleases. The evolution of Type II restriction-modification systems and their role in vivo are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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