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. 1986 Oct 24;14(20):7915–7927. doi: 10.1093/nar/14.20.7915

Tn7 transposition: a multigene process. Identification of a regulatory gene product.

G M Smith, P Jones
PMCID: PMC311824  PMID: 3022239

Abstract

Tn7 transposition is abolished by the deletion of a 2.2kb HindIII fragment from the central region of the transposon. Transposition is restored when the fragment is present in trans. When this fragment is present in trans with wild-type Tn7, transposition frequencies are stimulated 10-100-fold. The DNA sequence of this fragment has been determined and found to contain one long open reading frame coding for a protein of molecular weight 61 187. We have visualised this protein using a DNA-directed prokaryotic transcription-translation system. This gene may fill a regulatory role in the mechanism of Tn7 transposition. When present in trans the 2.2kb HindIII fragment alleviates a transcriptional block of promoter activity detected in Tn7.

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Selected References

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