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. 1986 Nov 11;14(21):8279–8290. doi: 10.1093/nar/14.21.8279

Green light induces transcription of the phycoerythrin operon in the cyanobacterium Calothrix 7601.

D Mazel, G Guglielmi, J Houmard, W Sidler, D A Bryant, N Tandeau de Marsac
PMCID: PMC311859  PMID: 2431391

Abstract

Phycobilisomes, the major light-harvesting complexes of cyanobacteria are multimolecular structures made up of chromophoric proteins called phycobiliproteins and non chromophoric linker polypeptides. We report here the isolation and nucleotide sequence of the genes, cpeA and cpeB, which in Calothrix PCC 7601 encode the alpha and beta subunits of phycoerythrin, one of the major phycobiliproteins. In Calothrix PCC 7601, modulation of the polypeptide composition of the phycobilisomes occurs in response to changes of the light wavelength, a phenomenon known as complementary chromatic adaptation. Under green illumination, cells synthesize phycoerythrin and its two specifically associated linker polypeptides (LR35 and LR36), while under red illumination none of these proteins are detected. Using specific probes, a single transcript (1450 nucleotide long) corresponding to the cpe genes was detected but only in green-light-grown cells, establishing the occurrence of transcriptional regulation for the expression of this operon in response to light wavelength changes. The size of this transcript excludes the possibility that the phycoerythrin-associated LR35 and LR36 could be cotranscribed with the cpeA and cpeB genes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bryant D. A. The photoregulated expression of multiple phycocyanin species. A general mechanism for the control of phycocyanin synthesis in chromatically adapting cyanobacteria. Eur J Biochem. 1981 Oct;119(2):425–429. doi: 10.1111/j.1432-1033.1981.tb05625.x. [DOI] [PubMed] [Google Scholar]
  2. Conley P. B., Lemaux P. G., Grossman A. R. Cyanobacterial light-harvesting complex subunits encoded in two red light-induced transcripts. Science. 1985 Nov 1;230(4725):550–553. doi: 10.1126/science.3931221. [DOI] [PubMed] [Google Scholar]
  3. Gendel S., Ohad I., Bogorad L. Control of Phycoerythrin Synthesis during Chromatic Adaptation. Plant Physiol. 1979 Nov;64(5):786–790. doi: 10.1104/pp.64.5.786. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Glatron M. F., Rapoport G. Biosynthesis of the parasporal inclusion of Bacillus thuringiensis: half-life of its corresponding messenger RNA. Biochimie. 1972;54(10):1291–1301. doi: 10.1016/s0300-9084(72)80070-1. [DOI] [PubMed] [Google Scholar]
  5. Glazer A. N. Light harvesting by phycobilisomes. Annu Rev Biophys Biophys Chem. 1985;14:47–77. doi: 10.1146/annurev.bb.14.060185.000403. [DOI] [PubMed] [Google Scholar]
  6. Lammers P. J., Haselkorn R. Sequence of the nifD gene coding for the alpha subunit of dinitrogenase from the cyanobacterium Anabaena. Proc Natl Acad Sci U S A. 1983 Aug;80(15):4723–4727. doi: 10.1073/pnas.80.15.4723. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Shinozaki K., Sugiura M. The gene for the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase is located close to the gene for the large subunit in the cyanobacterium Anacystis nidulans 6301. Nucleic Acids Res. 1983 Oct 25;11(20):6957–6964. doi: 10.1093/nar/11.20.6957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sidler W., Kumpf B., Rüdiger W., Zuber H. The complete amino-acid sequence of C-phycoerythrin from the cyanobacterium Fremyella diplosiphon. Biol Chem Hoppe Seyler. 1986 Jul;367(7):627–642. doi: 10.1515/bchm3.1986.367.2.627. [DOI] [PubMed] [Google Scholar]
  10. Tandeau de Marsac N., Mazel D., Bryant D. A., Houmard J. Molecular cloning and nucleotide sequence of a developmentally regulated gene from the cyanobacterium Calothrix PCC 7601: a gas vesicle protein gene. Nucleic Acids Res. 1985 Oct 25;13(20):7223–7236. doi: 10.1093/nar/13.20.7223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Vogelstein B., Gillespie D. Preparative and analytical purification of DNA from agarose. Proc Natl Acad Sci U S A. 1979 Feb;76(2):615–619. doi: 10.1073/pnas.76.2.615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. de Lorimier R., Bryant D. A., Porter R. D., Liu W. Y., Jay E., Stevens S. E., Jr Genes for the alpha and beta subunits of phycocyanin. Proc Natl Acad Sci U S A. 1984 Dec;81(24):7946–7950. doi: 10.1073/pnas.81.24.7946. [DOI] [PMC free article] [PubMed] [Google Scholar]

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