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. 1986 Nov 25;14(22):8755–8770. doi: 10.1093/nar/14.22.8755

Identification of estrogen-responsive DNA sequences by transient expression experiments in a human breast cancer cell line.

A Seiler-Tuyns, P Walker, E Martinez, A M Mérillat, F Givel, W Wahli
PMCID: PMC311909  PMID: 3466137

Abstract

The expression of a hybrid gene formed by the promoter region of the Xenopus laevis vitellogenin gene B1 and the CAT coding region is regulated by estrogen when the gene is transfected into hormone-responsive MCF-7 cells. Furthermore, the 5' flanking region of the gene B1 alone can confer inducibility to heterologous promoters, although to a varying extent depending on the promoter used. Deletion mapping of he vitellogenin hormone-responsive sequences revealed that a 13 bp element 5'-AGTCACTGTGACC-3' at position -334 is essential for estrogen inducibility. We have shown previously that this 13 bp element is present upstream of several liver-specific estrogen-inducible genes.

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Selected References

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