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. 1986 Dec 9;14(23):9491–9508. doi: 10.1093/nar/14.23.9491

Kinetics of the proton-deuteron exchange at position H8 of adenine and guanine in DNA.

R Brandes, A Ehrenberg
PMCID: PMC311972  PMID: 3025816

Abstract

Proton-NMR has been used to determine the activation energies and pre-exponential factors for the deuterium exchange of AH8 in poly(dA-dT).poly(dA-dT), and for GH8 in poly(dG-dC).poly(dG-dC). No simple relationship between the kinetic parameters and molecular conformation was found. By addition of 4.5 M NaCl a transition from the B to the Z conformation was induced for poly(dG-dC).poly(dG-dC), and an increased exchange rate was observed. The exchange rate for poly(dA-dT).poly(dA-dT) also increased below 64 degrees C, and a significant decrease in activation energy on addition of 4.5 M NaCl was observed. The exchange rates at T = 55.8 degrees C were also measured for the AH8 and GH8 in random sequence calf thymus DNA. From the difference in exchange rates, a method of preferential labeling of either the AH8 or the GH8 in high molecular weight DNA is evaluated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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