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. 1992 Mar 25;20(6):1265–1269. doi: 10.1093/nar/20.6.1265

Chemical synthesis of RNA using fast oligonucleotide deprotection chemistry.

R Vinayak 1, P Anderson 1, C McCollum 1, A Hampel 1
PMCID: PMC312168  PMID: 1373236

Abstract

The exocyclic amine protecting groups in oligonucleotide synthesis which require 8-16 hours at 55 degrees C for deprotection in ammonia have been replaced with more labile base protecting groups (dimethylformamidine for adenine and guanine and isobutyryl for cytosine). Using these fast oligonucleotide deprotecting groups which require 2-3 hours at 55 degrees C for complete deprotection, a new set of cyanoethyl phosphoramidite ribonucleoside monomers and supports has been developed. Ribozymes and substrate RNAs which were synthesized with these phosphoramidites were assayed and were found to have full catalytic (biological) activity.

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Selected References

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