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. 1992 Apr 25;20(8):1871–1877. doi: 10.1093/nar/20.8.1871

Cis-regulation of the L-type pyruvate kinase gene promoter by glucose, insulin and cyclic AMP.

M O Bergot 1, M J Diaz-Guerra 1, N Puzenat 1, M Raymondjean 1, A Kahn 1
PMCID: PMC312300  PMID: 1315961

Abstract

The glucose/insulin response element of the L-pyruvate kinase gene is a perfect palindrome located from nt -168 to -144 with respect to the cap site. This element (L4) is partially homologous to MLTF binding sites. Its full efficiency requires cooperation with a contiguous binding site for HNF4, termed L3 and located from nt -145 to -125. In the presence of the L4 element contiguous to L3, cyclic AMP inhibits activity of the L-PK promoter while in its absence, or when the normal L4-L3 contiguity is modified, cyclic AMP behaves as a transcriptional activator that does not seem to be sequence-specific. Therefore, we propose that the mechanism of inhibition of the L-PK gene by cyclic AMP requires precise interactions between the nucleoprotein complex built up at sites L4 and L3 and other components of the L-PK transcription initiation complex.

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