Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1989 Feb;57(2):590–595. doi: 10.1128/iai.57.2.590-595.1989

Endotoxin-induced serum factor that stimulates gamma interferon production.

K Nakamura 1, H Okamura 1, M Wada 1, K Nagata 1, T Tamura 1
PMCID: PMC313137  PMID: 2492265

Abstract

Serum from Mycobacterium bovis BCG-infected mice that had been challenged with lipopolysaccharide (LPS) exhibited a marked ability to induce gamma interferon (IFN-gamma) in cultures of spleen cells of normal mice in the presence of interleukin-2 (IL-2). The inducing activity became detectable in the circulatory system about 90 min after LPS challenge, disappeared at around 5 h, and was observable upon 640x dilution of the serum. Addition of monoclonal anti-IL-2 receptor antibody to the culture inhibited the induction by the serum. The activity induced high levels of IFN-gamma even in nylon wool-nonadherent cells, while concanavalin A failed to do so. Serum from uninfected mice challenged with LPS contained no such activity. The molecular weight of the active substance, estimated by gel filtration, was about 70,000. There were pronounced differences among mouse strains in the activities of the sera prepared, which paralleled the amounts of IFN-gamma produced in vivo. However, the levels of IFN-gamma produced in whole spleen cells and in nylon wool-nonadherent cells from mice of various strains were the same when stimulated with competent serum. These results indicate the existence of an unidentified factor that induces IFN-gamma in cooperation with IL-2 in macrophage-depleted splenocytes. They also suggest that IFN-gamma production in vivo is not genetically controlled at the lymphocyte level but, rather, at the level of synthesis of the unknown factor.

Full text

PDF
592

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Antonelli G., Dianzani F., Van Damme J., Amicucci P., De Marco F., Cefaro A. A macrophage-derived factor different from interleukin 1 and able to induce interferon-gamma and lymphoproliferation in resting T lymphocytes. Cell Immunol. 1988 May;113(2):376–386. doi: 10.1016/0008-8749(88)90035-4. [DOI] [PubMed] [Google Scholar]
  2. Apte R. N., Ascher O., Pluznik D. H. Genetic analysis of generation of serum interferon by bacterial lipopolysaccharide. J Immunol. 1977 Dec;119(6):1898–1902. [PubMed] [Google Scholar]
  3. Basham T. Y., Merigan T. C. Recombinant interferon-gamma increases HLA-DR synthesis and expression. J Immunol. 1983 Apr;130(4):1492–1494. [PubMed] [Google Scholar]
  4. Billiau A. Interferons and inflammation. J Interferon Res. 1987 Oct;7(5):559–567. doi: 10.1089/jir.1987.7.559. [DOI] [PubMed] [Google Scholar]
  5. Blanchard D. K., Djeu J. Y., Klein T. W., Friedman H., Stewart W. E., 2nd Interferon-gamma induction by lipopolysaccharide: dependence on interleukin 2 and macrophages. J Immunol. 1986 Feb 1;136(3):963–970. [PubMed] [Google Scholar]
  6. Guyre P. M., Morganelli P. M., Miller R. Recombinant immune interferon increases immunoglobulin G Fc receptors on cultured human mononuclear phagocytes. J Clin Invest. 1983 Jul;72(1):393–397. doi: 10.1172/JCI110980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Handa K., Suzuki R., Matsui H., Shimizu Y., Kumagai K. Natural killer (NK) cells as a responder to interleukin 2 (IL 2). II. IL 2-induced interferon gamma production. J Immunol. 1983 Feb;130(2):988–992. [PubMed] [Google Scholar]
  8. Heremans H., Dijkmans R., Sobis H., Vandekerckhove F., Billiau A. Regulation by interferons of the local inflammatory response to bacterial lipopolysaccharide. J Immunol. 1987 Jun 15;138(12):4175–4179. [PubMed] [Google Scholar]
  9. Huygen K., Palfliet K. In vitro production of gamma interferon is dependent on the mouse genotype. J Interferon Res. 1983;3(1):129–137. doi: 10.1089/jir.1983.3.129. [DOI] [PubMed] [Google Scholar]
  10. Huygen K., Palfliet K. Strain variation in interferon gamma production of BCG-sensitized mice challenged with PPD. I. CBA/Ca mice are low producers in vivo, but high producers in vitro. Cell Immunol. 1983 Sep;80(2):329–334. doi: 10.1016/0008-8749(83)90121-1. [DOI] [PubMed] [Google Scholar]
  11. Kasahara T., Hooks J. J., Dougherty S. F., Oppenheim J. J. Interleukin 2-mediated immune interferon (IFN-gamma) production by human T cells and T cell subsets. J Immunol. 1983 Apr;130(4):1784–1789. [PubMed] [Google Scholar]
  12. Kawase I., Brooks C. G., Kuribayashi K., Olabuenaga S., Newman W., Gillis S., Henney C. S. Interleukin 2 induces gamma-interferon production: participation of macrophages and NK-like cells. J Immunol. 1983 Jul;131(1):288–292. [PubMed] [Google Scholar]
  13. Le J., Lin J. X., Henriksen-DeStefano D., Vilcek J. Bacterial lipopolysaccharide-induced interferon-gamma production: roles of interleukin 1 and interleukin 2. J Immunol. 1986 Jun 15;136(12):4525–4530. [PubMed] [Google Scholar]
  14. McGhee J. R., Michalek S. M., Moore R. N., Mergenhagen S. E., Rosenstreich D. L. Genetic control of in vivo sensitivity to lipopolysaccharide: evidence for codominant inheritance. J Immunol. 1979 May;122(5):2052–2058. [PubMed] [Google Scholar]
  15. Mukaida N., Kasahara T., Hosoi J., Shioiri-Nakano K., Kawai T. Effects of anti-Tac antibody on the response of large granular lymphocytes to interleukin-2. Immunology. 1986 Jan;57(1):137–143. [PMC free article] [PubMed] [Google Scholar]
  16. Nathan C. F., Murray H. W., Wiebe M. E., Rubin B. Y. Identification of interferon-gamma as the lymphokine that activates human macrophage oxidative metabolism and antimicrobial activity. J Exp Med. 1983 Sep 1;158(3):670–689. doi: 10.1084/jem.158.3.670. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Neta R., Salvin S. B. In vivo release of lymphokines in different strains of mice. Cell Immunol. 1980 Apr;51(1):173–178. doi: 10.1016/0008-8749(80)90247-6. [DOI] [PubMed] [Google Scholar]
  18. Neta R., Salvin S. B. Mechanisms in the in vivo release of lymphokines: relationship of high and low responsiveness to other parameters of the immune response. Infect Immun. 1981 Oct;34(1):160–165. doi: 10.1128/iai.34.1.160-165.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Okamura H., Kawaguchi K., Shoji K., Kawade Y. High-level induction of gamma interferon with various mitogens in mice pretreated with Propionibacterium acnes. Infect Immun. 1982 Nov;38(2):440–443. doi: 10.1128/iai.38.2.440-443.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Okamura H., Wada M., Nagata K., Tamura T., Shoji K. Induction of murine gamma interferon production by lipopolysaccharide and interleukin-2 in Propionibacterium acnes-induced peritoneal exudate cells. Infect Immun. 1987 Feb;55(2):335–341. doi: 10.1128/iai.55.2.335-341.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Reem G. H., Yeh N. H. Interleukin 2 regulates expression of its receptor and synthesis of gamma interferon by human T lymphocytes. Science. 1984 Jul 27;225(4660):429–430. doi: 10.1126/science.6429853. [DOI] [PubMed] [Google Scholar]
  22. Steeg P. S., Moore R. N., Johnson H. M., Oppenheim J. J. Regulation of murine macrophage Ia antigen expression by a lymphokine with immune interferon activity. J Exp Med. 1982 Dec 1;156(6):1780–1793. doi: 10.1084/jem.156.6.1780. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Tyring S. K., Lefkowitz S. S. Strain differences in production of murine interferons. Proc Soc Exp Biol Med. 1980 Sep;164(4):519–523. doi: 10.3181/00379727-164-40908. [DOI] [PubMed] [Google Scholar]
  24. Virelizier J. L. Murine genotype influences the in vitro production of gamma (immune) interferon. Eur J Immunol. 1982 Nov;12(11):988–990. doi: 10.1002/eji.1830121119. [DOI] [PubMed] [Google Scholar]
  25. Wada M., Okamura H., Nagata K., Shimoyama T., Kawade Y. Cellular mechanisms in in vivo production of gamma interferon induced by lipopolysaccharide in mice infected with Mycobacterium bovis BCG. J Interferon Res. 1985 Summer;5(3):431–443. doi: 10.1089/jir.1985.5.431. [DOI] [PubMed] [Google Scholar]
  26. Wilson A. B., Harris J. M., Coombs R. R. Interleukin-2-induced production of interferon-gamma by resting human T cells and large granular lymphocytes: requirement for accessory cell factors, including interleukin-1. Cell Immunol. 1988 Apr 15;113(1):130–142. doi: 10.1016/0008-8749(88)90012-3. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES