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. 1989 Mar;57(3):823–826. doi: 10.1128/iai.57.3.823-826.1989

Cariogenicity of a lactate dehydrogenase-deficient mutant of Streptococcus mutans serotype c in gnotobiotic rats.

R J Fitzgerald 1, B O Adams 1, H J Sandham 1, S Abhyankar 1
PMCID: PMC313183  PMID: 2917788

Abstract

A lactate dehydrogenase-deficient (Ldh-) mutant of a human isolate of Streptococcus mutans serotype c was tested in a gnotobiotic rat caries model. Compared with the wild-type Ldh-positive (Ldh+) strains, it was significantly (alpha less than or equal to 0.005) less cariogenic in experiments with two different sublines of Sprague-Dawley rats. The Ldh- mutant strain 044 colonized the oral cavity of the test animals to the same extent as its parent strain 041, although its initial implantation was slightly but not significantly (P greater than or equal to 0.2) less. Multiple oral or fecal samples plated on 2,3,5-triphenyltetrazolium indicator medium revealed no evidence of back mutation from Ldh- to Ldh+ in vivo. Both Ldh+ strain 041 and Ldh- strain 044 demonstrated bacteriocinlike activity in vitro against a number of human strains of mutans streptococci representing serotype a (S. cricetus) and serotypes c and e (S. mutans). Serotypes b (S. rattus) and f (S. mutans) and strains of S. mitior, S. sanguis, and S. salivarius were not inhibited. Thus, Ldh mutant strain 044 possesses a number of desirable traits that suggest it should be investigated further as a possible effector strain for replacement therapy of dental caries. These traits include its stability and low cariogenicity in the sensitive gnotobiotic rat caries model, its bacteriocinlike activity against certain other cariogenic S. mutans (but not against more inocuous indigenous oral streptococci), and the fact that it is a member of the most prevalent human serotype of cariogenic streptococci.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abhyankar S., Sandham H. J., Chan K. H. Serotype c Streptococcus mutans mutatable to lactate dehydrogenase deficiency. J Dent Res. 1985 Nov;64(11):1267–1271. doi: 10.1177/00220345850640110201. [DOI] [PubMed] [Google Scholar]
  2. Fitzgerald R. J., Adams B. O., Fitzgerald D. B., Knox K. W. Cariogenicity of human plaque lactobacilli in gnotobiotic rats. J Dent Res. 1981 May;60(5):919–926. doi: 10.1177/00220345810600051201. [DOI] [PubMed] [Google Scholar]
  3. GUSTAFSSON B. E. Lightweight stainless steel systems for rearing germfree animals. Ann N Y Acad Sci. 1959 May 8;78:17–28. doi: 10.1111/j.1749-6632.1959.tb53092.x. [DOI] [PubMed] [Google Scholar]
  4. Gibbons R. J., Qureshi J. V. Virulence-related physiological changes and antigenic variation in populations of Streptococcus mutans colonizing gnotobiotic rats. Infect Immun. 1980 Sep;29(3):1082–1091. doi: 10.1128/iai.29.3.1082-1091.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hillman J. D. Lactate dehydrogenase mutants of Streptococcus mutans: isolation and preliminary characterization. Infect Immun. 1978 Jul;21(1):206–212. doi: 10.1128/iai.21.1.206-212.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ikeda T., Sandham H. J., Bradley E. L., Jr Changes in Streptococcus mutans and lactobacilli in plaque in relation to the initiation of dental caries in Negro children. Arch Oral Biol. 1973 Apr;18(4):555–566. doi: 10.1016/0003-9969(73)90076-9. [DOI] [PubMed] [Google Scholar]
  7. Johnson C. P., Gross S. M., Hillman J. D. Cariogenic potential in vitro in man and in vivo in the rat of lactate dehydrogenase mutants of Streptococcus mutans. Arch Oral Biol. 1980;25(11-12):707–713. doi: 10.1016/0003-9969(80)90124-7. [DOI] [PubMed] [Google Scholar]
  8. Johnson K. P., Hillman J. D. Competitive properties of lactate dehydrogenase mutants of the oral bacterium Streptococcus mutans in the rat. Arch Oral Biol. 1982;27(6):513–516. doi: 10.1016/0003-9969(82)90093-0. [DOI] [PubMed] [Google Scholar]
  9. KEYES P. H. Dental caries in the molar teeth of rats. II. A method for diagnosing and scoring several types of lesions simultaneously. J Dent Res. 1958 Nov-Dec;37(6):1088–1099. doi: 10.1177/00220345580370060901. [DOI] [PubMed] [Google Scholar]
  10. KEYES P. H., JORDAN H. V. PERIODONTAL LESIONS IN THE SYRIAN HAMSTER. III. FINDINGS RELATED TO AN INFECTIOUS AND TRANSMISSIBLE COMPONENT. Arch Oral Biol. 1964 Jul-Aug;9:377–400. doi: 10.1016/0003-9969(64)90024-x. [DOI] [PubMed] [Google Scholar]
  11. Loesche W. J. Role of Streptococcus mutans in human dental decay. Microbiol Rev. 1986 Dec;50(4):353–380. doi: 10.1128/mr.50.4.353-380.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mao M. W., Rosen S. Cariogenicity of mutants of Streptococcus mutans. J Dent Res. 1980 Oct;59(10):1620–1626. doi: 10.1177/00220345800590101101. [DOI] [PubMed] [Google Scholar]
  13. Salem H. H., Sandham H. J., Chan K. H. Lactate dehydrogenase-deficient mutants of serotype g Streptococcus mutans. J Dent Res. 1985 Oct;64(10):1191–1194. doi: 10.1177/00220345850640100201. [DOI] [PubMed] [Google Scholar]
  14. Thomson L. A., Little W. A., Bowen W. H., Sierra L. I., Aguirrer M., Gillespie G. Prevalence of Streptococcus mutans serotypes, Actinomyces, and other bacteria in the plaque of children. J Dent Res. 1980 Oct;59(10):1581–1589. doi: 10.1177/00220345800590100501. [DOI] [PubMed] [Google Scholar]

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