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. 1989 Aug;57(8):2475–2480. doi: 10.1128/iai.57.8.2475-2480.1989

Cellular immune responses of leprosy contacts to fractionated Mycobacterium leprae antigens.

S P Lee 1, N G Stoker 1, K A Grant 1, Z T Handzel 1, R Hussain 1, K P McAdam 1, H M Dockrell 1
PMCID: PMC313473  PMID: 2663725

Abstract

Antigens of armadillo-derived Mycobacterium leprae sonic extract were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and blotted onto a nitrocellulose membrane, and the unstained blot was converted into 20 fractions of antigen-bearing particles. These were tested in cellular proliferation assays, and reproducible results were obtained between batches of fractions. Peripheral blood mononuclear cells from healthy contacts of leprosy patients (presumed to have protective immunity) were tested with the fractions to investigate which antigens they recognized. A small group of tuberculoid leprosy patients were also tested. Both groups showed a wide range of responses. Almost every fraction stimulated proliferation with at least one donor, yet none was clearly immunodominant or inhibitory in either group. Thus, protective immunity did not appear to be associated with proliferation caused by any single fraction.

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Selected References

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  1. Abe M., Minagawa F., Yoshino Y., Ozawa T., Saikawa K., Saito T. Fluorescent leprosy antibody absorption (FLA-ABS) test for detecting subclinical infection with Mycobacterium leprae. Int J Lepr Other Mycobact Dis. 1980 Jun;48(2):109–119. [PubMed] [Google Scholar]
  2. Abou-Zeid C., Filley E., Steele J., Rook G. A. A simple new method for using antigens separated by polyacrylamide gel electrophoresis to stimulate lymphocytes in vitro after converting bands cut from Western blots into antigen-bearing particles. J Immunol Methods. 1987 Apr 2;98(1):5–10. doi: 10.1016/0022-1759(87)90429-7. [DOI] [PubMed] [Google Scholar]
  3. Bloom B. R., Godal T. Selective primary health care: strategies for control of disease in the developing world. V. Leprosy. Rev Infect Dis. 1983 Jul-Aug;5(4):765–780. doi: 10.1093/clinids/5.4.765. [DOI] [PubMed] [Google Scholar]
  4. Britton W. J., Garsia R. J., Hellqvist L., Watson J. D., Basten A. The characterization and immunoreactivity of a 70 kD protein common to Mycobacterium leprae and Mycobacterium bovis (BCG). Lepr Rev. 1986 Dec;57 (Suppl 2):67–75. [PubMed] [Google Scholar]
  5. Britton W. J., Hellqvist L., Basten A., Raison R. L. Mycobacterium leprae antigens involved in human immune responses. I. Identification of four antigens by monoclonal antibodies. J Immunol. 1985 Dec;135(6):4171–4177. [PubMed] [Google Scholar]
  6. Chakrabarty A. K., Maire M. A., Lambert P. H. SDS-PAGE analysis of M. leprae protein antigens reacting with antibodies from sera from lepromatous patients and infected armadillos. Clin Exp Immunol. 1982 Sep;49(3):523–531. [PMC free article] [PubMed] [Google Scholar]
  7. Converse P. J., Ottenhoff T. H., Gebre N., Ehrenberg J. P., Kiessling R. Cellular, humoral, and gamma interferon responses to Mycobacterium leprae and BCG antigens in healthy individuals exposed to leprosy. Scand J Immunol. 1988 May;27(5):515–525. doi: 10.1111/j.1365-3083.1988.tb02378.x. [DOI] [PubMed] [Google Scholar]
  8. Dockrell H. M., Stoker N. G., Lee S. P., Jackson M., Grant K. A., Jouy N. F., Lucas S. B., Hasan R., Hussain R., McAdam K. P. T-cell recognition of the 18-kilodalton antigen of Mycobacterium leprae. Infect Immun. 1989 Jul;57(7):1979–1983. doi: 10.1128/iai.57.7.1979-1983.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ehrenberg J. P., Gebre N. Analysis of the antigenic profile of Mycobacterium leprae: cross-reactive and unique specificities of human and rabbit antibodies. Scand J Immunol. 1987 Dec;26(6):673–681. doi: 10.1111/j.1365-3083.1987.tb02303.x. [DOI] [PubMed] [Google Scholar]
  10. Godal T. Growing points in leprosy research. 3. Immunological detection of sub-clinical infection in leprosy. Lepr Rev. 1974 Mar;45(1):22–30. doi: 10.5935/0305-7518.19740006. [DOI] [PubMed] [Google Scholar]
  11. Ivanyi J., Sinha S., Aston R., Cussell D., Keen M., Sengupta U. Definition of species specific and cross-reactive antigenic determinants of Mycobacterium leprae using monoclonal antibodies. Clin Exp Immunol. 1983 Jun;52(3):528–536. [PMC free article] [PubMed] [Google Scholar]
  12. Kaplan G., Gandhi R. R., Weinstein D. E., Levis W. R., Patarroyo M. E., Brennan P. J., Cohn Z. A. Mycobacterium leprae antigen-induced suppression of T cell proliferation in vitro. J Immunol. 1987 May 1;138(9):3028–3034. [PubMed] [Google Scholar]
  13. Kaufmann S. H., Väth U., Thole J. E., Van Embden J. D., Emmrich F. Enumeration of T cells reactive with Mycobacterium tuberculosis organisms and specific for the recombinant mycobacterial 64-kDa protein. Eur J Immunol. 1987 Mar;17(3):351–357. doi: 10.1002/eji.1830170308. [DOI] [PubMed] [Google Scholar]
  14. Klatser P. R., Hartskeerl R. A., van Schooten W. C., Kolk A. H., van Rens M. M., de Wit M. Y. Characterization of the 36 K antigen of Mycobacterium leprae. Lepr Rev. 1986 Dec;57 (Suppl 2):77–81. doi: 10.5935/0305-7518.19860057. [DOI] [PubMed] [Google Scholar]
  15. Klatser P. R., van Rens M. M., Eggelte T. A. Immunochemical characterization of Mycobacterium leprae antigens by the SDS-polyacrylamide gel electrophoresis immunoperoxidase technique (SGIP) using patients' sera. Clin Exp Immunol. 1984 Jun;56(3):537–544. [PMC free article] [PubMed] [Google Scholar]
  16. Kolk A. H., Ho M. L., Klatser P. R., Eggelte T. A., Kuijper S., de Jonge S., van Leeuwen J. Production and characterization of monoclonal antibodies to Mycobacterium tuberculosis, M. bovis (BCG) and M. leprae. Clin Exp Immunol. 1984 Dec;58(3):511–521. [PMC free article] [PubMed] [Google Scholar]
  17. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  18. Mustafa A. S., Gill H. K., Nerland A., Britton W. J., Mehra V., Bloom B. R., Young R. A., Godal T. Human T-cell clones recognize a major M. leprae protein antigen expressed in E. coli. Nature. 1986 Jan 2;319(6048):63–66. doi: 10.1038/319063a0. [DOI] [PubMed] [Google Scholar]
  19. Ottenhoff T. H., Klatser P. R., Ivanyi J., Elferink D. G., de Wit M. Y., de Vries R. R. Mycobacterium leprae-specific protein antigens defined by cloned human helper T cells. Nature. 1986 Jan 2;319(6048):66–68. doi: 10.1038/319066a0. [DOI] [PubMed] [Google Scholar]
  20. Shinnick T. M., Sweetser D., Thole J., van Embden J., Young R. A. The etiologic agents of leprosy and tuberculosis share an immunoreactive protein antigen with the vaccine strain Mycobacterium bovis BCG. Infect Immun. 1987 Aug;55(8):1932–1935. doi: 10.1128/iai.55.8.1932-1935.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Van Schooten W. C., Ottenhoff T. H., Klatser P. R., Thole J., De Vries R. R., Kolk A. H. T cell epitopes on the 36K and 65K Mycobacterium leprae antigens defined by human T cell clones. Eur J Immunol. 1988 Jun;18(6):849–854. doi: 10.1002/eji.1830180604. [DOI] [PubMed] [Google Scholar]
  23. Vega-Lopez F., Stoker N. G., Locniskar M. F., Dockrell H. M., Grant K. A., McAdam K. P. Recognition of mycobacterial antigens by sera from patients with leprosy. J Clin Microbiol. 1988 Dec;26(12):2474–2479. doi: 10.1128/jcm.26.12.2474-2479.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Young D. B., Fohn M. J., Khanolkar S. R., Buchanan T. M. Monoclonal antibodies to a 28,000 mol. wt protein antigen of Mycobacterium leprae. Clin Exp Immunol. 1985 Jun;60(3):546–552. [PMC free article] [PubMed] [Google Scholar]
  25. Young D. B., Lamb J. R. T lymphocytes respond to solid-phase antigen: a novel approach to the molecular analysis of cellular immunity. Immunology. 1986 Oct;59(2):167–171. [PMC free article] [PubMed] [Google Scholar]
  26. Young D., Lathigra R., Hendrix R., Sweetser D., Young R. A. Stress proteins are immune targets in leprosy and tuberculosis. Proc Natl Acad Sci U S A. 1988 Jun;85(12):4267–4270. doi: 10.1073/pnas.85.12.4267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Young R. A., Mehra V., Sweetser D., Buchanan T., Clark-Curtiss J., Davis R. W., Bloom B. R. Genes for the major protein antigens of the leprosy parasite Mycobacterium leprae. Nature. 1985 Aug 1;316(6027):450–452. doi: 10.1038/316450a0. [DOI] [PubMed] [Google Scholar]

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