Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1989 Sep;57(9):2909–2911. doi: 10.1128/iai.57.9.2909-2911.1989

Hemagglutinating properties of Shigella dysenteriae type 1 and other Shigella species.

F Qadri 1, S Haq 1, I Ciznár 1
PMCID: PMC313547  PMID: 2503449

Abstract

Strains of Shigella dysenteriae type 1 cultured in Casamino Acids-yeast extract broth medium in the presence of 1 mM calcium chloride at 37 degrees C for 22 h induced hemagglutination of erythrocytes that was inhibited by N-acetylneuraminic acid, N-acetylneuramin-lactose, and alpha 1-glycoprotein. The hemagglutination was heat labile, and the absence of cell-surface appendages suggested a nonfimbrial adhesin(s). Under the same conditions, strains of Shigella flexneri (types 1a, 1b, 2a, and 2b) showed N-acetylneuraminic acid-resistant hemagglutination of erythrocytes.

Full text

PDF
2910

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahmed Z. U., Sarker M. R., Sack D. A. Nutritional requirements of shigellae for growth in a minimal medium. Infect Immun. 1988 Apr;56(4):1007–1009. doi: 10.1128/iai.56.4.1007-1009.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Atkinson H. M., Trust T. J. Hemagglutination properties and adherence ability of Aeromonas hydrophila. Infect Immun. 1980 Mar;27(3):938–946. doi: 10.1128/iai.27.3.938-946.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Beachey E. H. Bacterial adherence: adhesin-receptor interactions mediating the attachment of bacteria to mucosal surface. J Infect Dis. 1981 Mar;143(3):325–345. doi: 10.1093/infdis/143.3.325. [DOI] [PubMed] [Google Scholar]
  4. Binns M. M. Molecular genetics of virulence in Shigella. Microbiol Sci. 1985 Sep;2(9):275–278. [PubMed] [Google Scholar]
  5. Duguid J. P., Anderson E. S., Campbell I. Fimbriae and adhesive properties in Salmonellae. J Pathol Bacteriol. 1966 Jul;92(1):107–138. doi: 10.1002/path.1700920113. [DOI] [PubMed] [Google Scholar]
  6. Duguid J. P., Clegg S., Wilson M. I. The fimbrial and non-fimbrial haemagglutinins of Escherichia coli. J Med Microbiol. 1979 May;12(2):213–227. doi: 10.1099/00222615-12-2-213. [DOI] [PubMed] [Google Scholar]
  7. Evans D. G., Evans D. J., Jr, Clegg S., Pauley J. A. Purification and characterization of the CFA/I antigen of enterotoxigenic Escherichia coli. Infect Immun. 1979 Aug;25(2):738–748. doi: 10.1128/iai.25.2.738-748.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Evans D. G., Evans D. J., Jr, Tjoa W. Hemagglutination of human group A erythrocytes by enterotoxigenic Escherichia coli isolated from adults with diarrhea: correlation with colonization factor. Infect Immun. 1977 Nov;18(2):330–337. doi: 10.1128/iai.18.2.330-337.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Evans D. J., Jr, Evans D. G., DuPont H. L. Hemagglutination patterns of enterotoxigenic and enteropathogenic Escherichia coli determined with human, bovine, chicken, and guinea pig erythrocytes in the presence and absence of mannose. Infect Immun. 1979 Feb;23(2):336–346. doi: 10.1128/iai.23.2.336-346.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gaastra W., de Graaf F. K. Host-specific fimbrial adhesins of noninvasive enterotoxigenic Escherichia coli strains. Microbiol Rev. 1982 Jun;46(2):129–161. doi: 10.1128/mr.46.2.129-161.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Goldhar J., Perry R., Golecki J. R., Hoschutzky H., Jann B., Jann K. Nonfimbrial, mannose-resistant adhesins from uropathogenic Escherichia coli O83:K1:H4 and O14:K?:H11. Infect Immun. 1987 Aug;55(8):1837–1842. doi: 10.1128/iai.55.8.1837-1842.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gonzalez E. A., Blanco J., Baloda S. B., Wadström T. Relative cell surface hydrophobicity of Escherichia coli strains with various recognized fimbrial antigens and without recognized fimbriae. Zentralbl Bakteriol Mikrobiol Hyg A. 1988 Aug;269(2):218–236. doi: 10.1016/s0176-6724(88)80100-7. [DOI] [PubMed] [Google Scholar]
  13. Hultgren S. J., Schwan W. R., Schaeffer A. J., Duncan J. L. Regulation of production of type 1 pili among urinary tract isolates of Escherichia coli. Infect Immun. 1986 Dec;54(3):613–620. doi: 10.1128/iai.54.3.613-620.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Iwanaga M., Yamamoto K. New medium for the production of cholera toxin by Vibrio cholerae O1 biotype El Tor. J Clin Microbiol. 1985 Sep;22(3):405–408. doi: 10.1128/jcm.22.3.405-408.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Izhar M., Nuchamowitz Y., Mirelman D. Adherence of Shigella flexneri to guinea pig intestinal cells is mediated by a mucosal adhesion. Infect Immun. 1982 Mar;35(3):1110–1118. doi: 10.1128/iai.35.3.1110-1118.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Levine M. M., Kaper J. B., Black R. E., Clements M. L. New knowledge on pathogenesis of bacterial enteric infections as applied to vaccine development. Microbiol Rev. 1983 Dec;47(4):510–550. doi: 10.1128/mr.47.4.510-550.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mulczyk M. Hemagglutinating properties of Shigella bacilli. Arch Immunol Ther Exp (Warsz) 1967;15(4):636–639. [PubMed] [Google Scholar]
  18. Old D. C. Inhibition of the interaction between fimbrial haemagglutinins and erythrocytes by D-mannose and other carbohydrates. J Gen Microbiol. 1972 Jun;71(1):149–157. doi: 10.1099/00221287-71-1-149. [DOI] [PubMed] [Google Scholar]
  19. Osada Y., Une T., Ikeuchi T., Ogawa H. Effect of calcium on the cell infectivity of virulent Shigella flexneri 2a. Jpn J Microbiol. 1974 Jul;18(4):321–326. doi: 10.1111/j.1348-0421.1974.tb00816.x. [DOI] [PubMed] [Google Scholar]
  20. Qadri F., Hossain S. A., Ciznár I., Haider K., Ljungh A., Wadstrom T., Sack D. A. Congo red binding and salt aggregation as indicators of virulence in Shigella species. J Clin Microbiol. 1988 Jul;26(7):1343–1348. doi: 10.1128/jcm.26.7.1343-1348.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Ramphal R., Pyle M. Evidence for mucins and sialic acid as receptors for Pseudomonas aeruginosa in the lower respiratory tract. Infect Immun. 1983 Jul;41(1):339–344. doi: 10.1128/iai.41.1.339-344.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. SERENY B. Experimental shigella keratoconjunctivitis; a preliminary report. Acta Microbiol Acad Sci Hung. 1955;2(3):293–296. [PubMed] [Google Scholar]
  23. Sjöberg P. O., Lindahl M., Porath J., Wadström T. Purification and characterization of CS2, a sialic acid-specific haemagglutinin of enterotoxigenic Escherichia coli. Biochem J. 1988 Oct 1;255(1):105–111. doi: 10.1042/bj2550105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Williams P. H., Knutton S., Brown M. G., Candy D. C., McNeish A. S. Characterization of nonfimbrial mannose-resistant protein hemagglutinins of two Escherichia coli strains isolated from infants with enteritis. Infect Immun. 1984 Jun;44(3):592–598. doi: 10.1128/iai.44.3.592-598.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES