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. 1989 Jul;57(7):2214–2222. doi: 10.1128/iai.57.7.2214-2222.1989

Cellular events and intracellular survival of Campylobacter jejuni during infection of HEp-2 cells.

M A De Melo 1, G Gabbiani 1, J C Pechère 1
PMCID: PMC313863  PMID: 2731988

Abstract

Invasion and intracellular survival of Campylobacter jejuni in HEp-2 cells were analyzed by transmission electron microscopy and by viable counts after killing of extracellular bacteria by gentamicin. During the first 30 min after challenge, no bacteria were seen in association with the host cell. After 1 h, campylobacters apparently attached to the cell membrane, with areas of close appositions. In these areas, an intracellular network of actin-like filaments was seen beneath the plasma membrane. Other bacteria were included into endocytic vacuoles. After 3 h, an intense lysosomal response was observed in the host cells, as determined by the presence of myelinic forms and acid phosphatase activity. After 9 h, bacteria still contained in vacuoles showed signs of degradation with a change from spiral to coccal forms. Morphological evidence of phagosome-lysosome fusion was also seen, and these observations by transmission electron microscopy correlated well with a decrease in bacteria viability 9 h after challenge, as determined from separate kinetics studies. Inhibitors of phagocytosis were observed to reduce markedly the entry of C. jejuni into the cells at concentrations which apparently did not affect bacterial viability. These results suggest that the campylobacters were successively attached to the HEp-2 cell membrane, internalized by a phagocytic-like mechanism, and digested after phagosome-lysosome fusion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bukholm G. Effect of cytochalasin B and dihydrocytochalasin B on invasiveness of entero-invasive bacteria in HEp-2 cell cultures. Acta Pathol Microbiol Immunol Scand B. 1984 Jun;92(3):145–149. doi: 10.1111/j.1699-0463.1984.tb02809.x. [DOI] [PubMed] [Google Scholar]
  2. Clerc P., Sansonetti P. J. Entry of Shigella flexneri into HeLa cells: evidence for directed phagocytosis involving actin polymerization and myosin accumulation. Infect Immun. 1987 Nov;55(11):2681–2688. doi: 10.1128/iai.55.11.2681-2688.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fauchere J. L., Rosenau A., Veron M., Moyen E. N., Richard S., Pfister A. Association with HeLa cells of Campylobacter jejuni and Campylobacter coli isolated from human feces. Infect Immun. 1986 Nov;54(2):283–287. doi: 10.1128/iai.54.2.283-287.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Field L. H., Headley V. L., Underwood J. L., Payne S. M., Berry L. J. The chicken embryo as a model for campylobacter invasion: comparative virulence of human isolates of Campylobacter jejuni and Campylobacter coli. Infect Immun. 1986 Oct;54(1):118–125. doi: 10.1128/iai.54.1.118-125.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hale T. L. Invasion of epithelial cells by shigellae. Ann Inst Pasteur Microbiol. 1986 May-Jun;137A(3):311–314. doi: 10.1016/s0769-2609(86)80040-0. [DOI] [PubMed] [Google Scholar]
  6. Hale T. L., Morris R. E., Bonventre P. F. Shigella infection of henle intestinal epithelial cells: role of the host cell. Infect Immun. 1979 Jun;24(3):887–894. doi: 10.1128/iai.24.3.887-894.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Humphrey C. D., Montag D. M., Pittman F. E. Experimental infection of hamsters with Campylobacter jejuni. J Infect Dis. 1985 Mar;151(3):485–493. doi: 10.1093/infdis/151.3.485. [DOI] [PubMed] [Google Scholar]
  8. Kiehlbauch J. A., Albach R. A., Baum L. L., Chang K. P. Phagocytosis of Campylobacter jejuni and its intracellular survival in mononuclear phagocytes. Infect Immun. 1985 May;48(2):446–451. doi: 10.1128/iai.48.2.446-451.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kihlström E., Nilsson L. Endocytosis of Salmonella typhimurium 395 MS and MR10 by HeLa cells. Acta Pathol Microbiol Scand B. 1977 Oct;85B(5):322–328. doi: 10.1111/j.1699-0463.1977.tb01982.x. [DOI] [PubMed] [Google Scholar]
  10. Popiel I., Turnbull P. C. Passage of Salmonella enteritidis and Salmonella thompson through chick ileocecal mucosa. Infect Immun. 1985 Mar;47(3):786–792. doi: 10.1128/iai.47.3.786-792.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sansonetti P. J., Ryter A., Clerc P., Maurelli A. T., Mounier J. Multiplication of Shigella flexneri within HeLa cells: lysis of the phagocytic vacuole and plasmid-mediated contact hemolysis. Infect Immun. 1986 Feb;51(2):461–469. doi: 10.1128/iai.51.2.461-469.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Small P. L., Isberg R. R., Falkow S. Comparison of the ability of enteroinvasive Escherichia coli, Salmonella typhimurium, Yersinia pseudotuberculosis, and Yersinia enterocolitica to enter and replicate within HEp-2 cells. Infect Immun. 1987 Jul;55(7):1674–1679. doi: 10.1128/iai.55.7.1674-1679.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Vesikari T., Bromirska J., Mäki M. Enhancement of invasiveness of Yersinia enterocolitica and Escherichia coli in HEp-2 cells by centrifugation. Infect Immun. 1982 May;36(2):834–836. doi: 10.1128/iai.36.2.834-836.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Walker R. I., Caldwell M. B., Lee E. C., Guerry P., Trust T. J., Ruiz-Palacios G. M. Pathophysiology of Campylobacter enteritis. Microbiol Rev. 1986 Mar;50(1):81–94. doi: 10.1128/mr.50.1.81-94.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Youssef M., Corthier G., Goossens H., Tancrede C., Henry-Amar M., Andremont A. Comparative translocation of enteropathogenic Campylobacter spp. and Escherichia coli from the intestinal tract of gnotobiotic mice. Infect Immun. 1987 Apr;55(4):1019–1021. doi: 10.1128/iai.55.4.1019-1021.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. de Melo M. A., Pechère J. C. Effect of mucin on Campylobacter jejuni association and invasion on HEp-2 cells. Microb Pathog. 1988 Jul;5(1):71–76. doi: 10.1016/0882-4010(88)90083-6. [DOI] [PubMed] [Google Scholar]

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