Abstract
Yotis, W. W. (Loyola University Medical School, Chicago, Ill.). Effect of the antibacterial serum factor on staphylococcal infections. J. Bacteriol. 83:137–143. 1962—Intracerebral injections of mice with 1 to 5 × 106 washed viable cells previously exposed for 1 hr at 4 C to 2 mg/ml of the serum factor resulted in 0 to 30% mortality when three recent isolates of yellow, hemolytic, coagulase-positive strains of Staphylococcus aureus were used. Mice inoculated in the same manner with the above strains, but exposed to an inactive preparation of the serum factor, showed a 60 to 90% mortality.
Addition of partially purified coagulase to the serum factor neutralized the protective action of the serum factor.
The serum factor was found primarily in the supernatant obtained following 62% (NH4)2SO4 saturation of the water-soluble globulin portion and precipitated by one-half volume of undiluted 95% ethanol. Plate counts, manometric techniques, and animal protection studies were employed to follow purification of the serum factor. If dry weight is taken as the criterion of purification, the active substance showed a 40-fold purification over a previous preparation of this substance.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BORDUAS A., FRAPPIER A., SONEA S. Action protectrice de la gamma globuline humaine contre une infection staphylococcique expérimentale. Union Med Can. 1956 Sep;85(9):1028–1032. [PubMed] [Google Scholar]
- EKSTEDT R. D. Further studies on the antibacterial activity of human serum on Micrococcus pyogenes and its inhibition by coagulase. J Bacteriol. 1956 Aug;72(2):157–161. doi: 10.1128/jb.72.2.157-161.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EKSTEDT R. D., NUNGESTER W. J. Coagulase in reversing antibacterial activity of normal human serum on Micrococcus pyogenes. Proc Soc Exp Biol Med. 1955 May;89(1):90–94. doi: 10.3181/00379727-89-21724. [DOI] [PubMed] [Google Scholar]
- EKSTEDT R. D. The effect of coagulase on the antibacterial activity of normal human serum against selected strains of Micrococcus pyogenes. Ann N Y Acad Sci. 1956 Aug 31;65(3):119–131. doi: 10.1111/j.1749-6632.1956.tb36630.x. [DOI] [PubMed] [Google Scholar]
- EKSTEDT R. D., YOTIS W. W. Studies on staphylococci. II. Effect of coagulase on the virulence of coagulase negative strains. J Bacteriol. 1960 Oct;80:496–500. doi: 10.1128/jb.80.4.496-500.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HARRIS J. R., SCHICK B. The use of gamma globulin in infection refractory to antibiotics. J Mt Sinai Hosp N Y. 1954 Sep-Oct;21(3):148–161. [PubMed] [Google Scholar]
- LAMBERT H. P. Gamma globulin in experimental staphylococcal infections. J Lab Clin Med. 1960 Nov;56:701–710. [PubMed] [Google Scholar]
- NETER E., GORZYNSKI E. A., DRISLANE A. M., HARRIS A. H., RAJNOVICH E. Detection of staphylococcal antibodies in human gamma globulin and serum by hemagglutination tests. Proc Soc Exp Biol Med. 1959 Jul;101(3):484–487. doi: 10.3181/00379727-101-24990. [DOI] [PubMed] [Google Scholar]
- WELD J. T., ROGERS D. E. Staphylococcal immunity: production of staphylococcal hemagglutinins in rabbits receiving staphylococcal vaccine. Proc Soc Exp Biol Med. 1960 Feb;103:311–314. doi: 10.3181/00379727-103-25501. [DOI] [PubMed] [Google Scholar]
- YOTIS W. W., EKSTEDT R. D. Studies on staphylococci. I. Effect of serum and coagulase on the metabolism of coagulase positive and coagulase negative strains. J Bacteriol. 1959 Oct;78:567–574. doi: 10.1002/path.1700780225. [DOI] [PMC free article] [PubMed] [Google Scholar]
- YOTIS W. W., EKSTEDT R. D. Studies on staphylococci. III. Further studies on purification and mechanism of action of an antibacterial human serum factor. J Bacteriol. 1960 Nov;80:719–725. doi: 10.1128/jb.80.5.719-725.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]