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. 1967 Jan;93(1):221–229. doi: 10.1128/jb.93.1.221-229.1967

Complex Lipids of Rhodomicrobium vannielii1

Chong-Eel Park a,2, Leslie R Berger a
PMCID: PMC314992  PMID: 6020408

Abstract

Eight components, seven of which contained phosphorus, were found in the phospholipid fraction of Rhodomicrobium vannielii. The major components were lipoamino acid (o-ornithine ester of phosphatidyl glycerol, 46.5%) and phosphatidyl choline (26.5%). The other six components were phosphatidyl glycerol (9.7%), bisphosphatidic acid (6.7%), phosphatidyl ethanolamine (4.5%), phosphatidic acid (1.8%), lysophosphatidyl glycerol-o-ornithine ester (3.2%), and N,N-ornithine amide of unidentified fatty acid (0.95%). Total phospholipid accounted for 4.2% of cell dry weight. The major fatty acid was vaccenic acid, C18:1, which accounted for approximately 90% of the total fatty acids of the complex lipid fraction. The other four fatty acids were C16:0 (6.25%), C18:0 (3.8%), C14:0 (0.7%), and C16:1 (0.35%). The sulfolipid content was 0.01% of the cell dry weight or 0.14 μmoles per g of dried cells, assuming that its fatty acid component is vaccenic acid. No steroids were detected.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABRAMSON D., BLECHER M. QUANTITATIVE TWO-DIMENSIONAL THIN-LAYER CHROMATOGRAPHY OF NATURALLY OCCURRING PHOSPHOLIPIDS. J Lipid Res. 1964 Oct;5:628–631. [PubMed] [Google Scholar]
  2. BARTLETT G. R. Phosphorus assay in column chromatography. J Biol Chem. 1959 Mar;234(3):466–468. [PubMed] [Google Scholar]
  3. Bard R. C., McClung L. S. Biochemical Properties of the Toxins of Clostridium novyi and Clostridium hemolyticum. J Bacteriol. 1948 Nov;56(5):665–670. doi: 10.1128/jb.56.5.665-670.1948. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DAWSON R. M. A hydrolytic procedure for the identification and estimation of individual phospholipids in biological samples. Biochem J. 1960 Apr;75:45–53. doi: 10.1042/bj0750045. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DAWSON R. M., HEMINGTON N., DAVENPORT J. B. Improvements in the method of determining individual phospholipids in a complex mixture by successive chemical hydrolyses. Biochem J. 1962 Sep;84:497–501. doi: 10.1042/bj0840497. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  7. GOLDFINE H., ELLIS M. E. N-METHYL GROUPS IN BACTERIAL LIPIDS. J Bacteriol. 1964 Jan;87:8–15. doi: 10.1128/jb.87.1.8-15.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. GOLDFINE H. The characterization and biosynthesis of an N-methylethanolamine phospholipid from Clostridium butyricum. Biochim Biophys Acta. 1962 May 21;59:504–506. doi: 10.1016/0006-3002(62)90212-3. [DOI] [PubMed] [Google Scholar]
  9. GORCHEIN A. ORNITHINE IN RHODOPSEUDOMONAS SPHEROIDES. Biochim Biophys Acta. 1964 Jun 15;84:356–358. doi: 10.1016/0926-6542(64)90064-2. [DOI] [PubMed] [Google Scholar]
  10. HACK M. H. Analysis of lipids by spot tests on filter-paper disk chromatograms. Biochem J. 1953 Jul;54(4):602–605. doi: 10.1042/bj0540602. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HANES C. S., ISHERWOOD F. A. Separation of the phosphoric esters on the filter paper chromatogram. Nature. 1949 Dec 31;164(4183):1107-12, illust. doi: 10.1038/1641107a0. [DOI] [PubMed] [Google Scholar]
  12. HORNING E. C., AHRENS E. H., Jr, LIPSKY S. R., MATTSON F. H., MEAD J. F., TURNER D. A., GOLDWATER W. H. QUANTITATIVE ANALYSIS OF FATTY ACIDS BY GAS-LIQUID CHROMATOGRAPHY. J Lipid Res. 1964 Jan;5:20–27. [PubMed] [Google Scholar]
  13. HOUTSMULLER U. M., van DEENEN L. Identification of a bacterial phospholipid as an O-ornithine ester of phosphatidyl glycerol. Biochim Biophys Acta. 1963 Apr 23;70:211–213. doi: 10.1016/0006-3002(63)90743-1. [DOI] [PubMed] [Google Scholar]
  14. HUSTON C. K., ALBRO P. W., GRINDEY G. B. LIPIDS OF SARCINA LUTEA. 3. COMPOSITION OF THE COMPLEX LIPIDS. J Bacteriol. 1965 Mar;89:768–775. doi: 10.1128/jb.89.3.768-775.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. HUSTON C. K., ALBRO P. W. LIPIDS OF SARCINA LUTEA. I. FATTY ACID COMPOSITION OF THE EXTRACTABLE LIPIDS. J Bacteriol. 1964 Aug;88:425–432. doi: 10.1128/jb.88.2.425-432.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. IKAWA M. NATURE OF THE LIPIDS OF SOME LACTIC ACID BACTERIA. J Bacteriol. 1963 Apr;85:772–781. doi: 10.1128/jb.85.4.772-781.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. KATES M., ADAMS G. A., MARTIN S. M. LIPIDS OF SERRATIA MARCESCENS. Can J Biochem. 1964 Apr;42:461–479. doi: 10.1139/o64-054. [DOI] [PubMed] [Google Scholar]
  18. KATES M., SEHGAL S. N., GIBBONS N. E. The lipid composition of Micrococcus halodenitrificans as influenced by salt concentration. Can J Microbiol. 1961 Aug;7:427–435. doi: 10.1139/m61-052. [DOI] [PubMed] [Google Scholar]
  19. LANEELLE M. A., LANEELLE G., ASSELINEAU J. [On the presence of ornithine in bacterial lipids]. Biochim Biophys Acta. 1963 Feb 19;70:99–101. doi: 10.1016/0006-3002(63)90727-3. [DOI] [PubMed] [Google Scholar]
  20. LASCELLES J., SZILAGYI J. F. PHOSPHOLIPID SYNTHESIS BY RHODOPSEUDOMONAS SPHEROIDES IN RELATION TO THE FORMATION OF PHOTOSYNTHETIC PIGMENTS. J Gen Microbiol. 1965 Jan;38:55–64. doi: 10.1099/00221287-38-1-55. [DOI] [PubMed] [Google Scholar]
  21. LEPAGE M. THE SEPARATION AND IDENTIFICATION OF PLANT PHOSPHOLIPIDS AND GLYCOLIPIDS BY TWO-DIMENSIONAL THIN-LAYER CHROMATOGRAPHY. J Chromatogr. 1964 Jan;13:99–103. doi: 10.1016/s0021-9673(01)95078-2. [DOI] [PubMed] [Google Scholar]
  22. MATCHES J. R., WALKER H. W., AYRES J. C. PHOSPHOLIPIDS IN VEGETATIVE CELLS AND SPORES OF BACILLUS POLYMYXA. J Bacteriol. 1964 Jan;87:16–23. doi: 10.1128/jb.87.1.16-23.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. MORRISON W. R., SMITH L. M. PREPARATION OF FATTY ACID METHYL ESTERS AND DIMETHYLACETALS FROM LIPIDS WITH BORON FLUORIDE--METHANOL. J Lipid Res. 1964 Oct;5:600–608. [PubMed] [Google Scholar]
  24. O'Brien J. S., Benson A. A. Isolation and fatty acid composition of the plant sulfolipid and galactolipids. J Lipid Res. 1964 Jul;5(3):432–434. [PubMed] [Google Scholar]
  25. RAPPORT M. M., ALONZO N. Photometric determination of fatty acid ester groups in phospholipides. J Biol Chem. 1955 Nov;217(1):193–198. [PubMed] [Google Scholar]
  26. SEHGAL S. N., KATES M., GIBBONS N. E. Lipids of Halobacterium cutirubrum. Can J Biochem Physiol. 1962 Jan;40:69–81. [PubMed] [Google Scholar]
  27. SPERRY W. M., WEBB M. A revision of the Schoenheimer-Sperry method for cholesterol determination. J Biol Chem. 1950 Nov;187(1):97–106. [PubMed] [Google Scholar]
  28. STANIER R. Y. [The place of bacteria in the living world]. Ann Inst Pasteur (Paris) 1961 Sep;101:297–312. [PubMed] [Google Scholar]
  29. WHEELDON L. W., COLLINS F. D. Studies on phospholipids. I. The determination of amino nitrogen in unhydrolysed phospholipids. Biochem J. 1957 Jul;66(3):435–441. doi: 10.1042/bj0660435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Wood B. J., Nichols B. W., James A. T. The lipids and fatty acid metabolism of photosynthetic bacteria. Biochim Biophys Acta. 1965 Oct 4;106(2):261–273. doi: 10.1016/0005-2760(65)90034-2. [DOI] [PubMed] [Google Scholar]
  31. den Kamp JA O. P., Houtsmuller U. M., van Deenen L. L. On the phospholipids of Bacillus megaterium. Biochim Biophys Acta. 1965 Oct 4;106(2):438–441. doi: 10.1016/0005-2760(65)90059-7. [DOI] [PubMed] [Google Scholar]

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