Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1967 Aug;94(2):333–343. doi: 10.1128/jb.94.2.333-343.1967

Identification of Fatty Acids and Aliphatic Hydrocarbons in Sarcina lutea by Gas Chromatography and Combined Gas Chromatography-Mass Spectrometry

T G Tornabene 1,2,1, E Gelpi 1,2, J Oró 1,2
PMCID: PMC315045  PMID: 6039356

Abstract

The composition and nature of the fatty acids and hydrocarbons of Sarcina lutea were elucidated by gas chromatography and by combined gas chromatography-mass spectrometry. The distribution of fatty acids found in S. lutea showed two families of pairs, or dyads, of saturated monocarboxylic acids (C12–C18) with and without methyl branching. These pairs of fatty acids showed a pattern of iso and anteiso structures for C13, C15, and C17, and iso and normal structures for C12, C14, and C16. Only the C18 showed unsaturation. The distribution of hydrocarbons in the range C22–C29 showed two families of tetrads of unsaturated aliphatic hydrocarbons all showing methyl branching. Each tetrad was composed of four isomers identified as two iso olefins and two anteiso olefins. The only difference between the tetrads pertaining to different families was found in the relative gas chromatographic retention times of the last two components of each group.

Full text

PDF
337

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALBRO P. W., HUSTON C. K. LIPIDS OF SARCINA LUTEA. II. HYDROCARBON CONTENT OF THE LIPID EXTRACTS. J Bacteriol. 1964 Oct;88:981–986. doi: 10.21236/ad0437968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brown O. R., Clark J. B. Pigment of Nocardia corallina. J Bacteriol. 1966 Dec;92(6):1844–1845. doi: 10.1128/jb.92.6.1844-1845.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carr F. H., Price E. A. Colour Reactions Attributed to Vitamin A. Biochem J. 1926;20(3):497–501. doi: 10.1042/bj0200497. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. HUSTON C. K., ALBRO P. W., GRINDEY G. B. LIPIDS OF SARCINA LUTEA. 3. COMPOSITION OF THE COMPLEX LIPIDS. J Bacteriol. 1965 Mar;89:768–775. doi: 10.1128/jb.89.3.768-775.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HUSTON C. K., ALBRO P. W. LIPIDS OF SARCINA LUTEA. I. FATTY ACID COMPOSITION OF THE EXTRACTABLE LIPIDS. J Bacteriol. 1964 Aug;88:425–432. doi: 10.1128/jb.88.2.425-432.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jensen S. L. Biosynthesis and function of carotenoid pigments in microorganisms. Annu Rev Microbiol. 1965;19:163–182. doi: 10.1146/annurev.mi.19.100165.001115. [DOI] [PubMed] [Google Scholar]
  7. Kleinkauf H. Carotine und Carotinoidsynthese in Rhizobium lupini-Mutanten. Arch Mikrobiol. 1966 Mar 8;53(2):154–158. [PubMed] [Google Scholar]
  8. Liaaen-Jensen S., Weeks O. B., Strang R. H., Thirkell D. Identity of the C-50-carotenoid dehydrogenans-P439 and sarcinaxanthin. Nature. 1967 Apr 22;214(5086):379–380. doi: 10.1038/214379c0. [DOI] [PubMed] [Google Scholar]
  9. NESBITT J. A., 3rd, LENNARZ W. J. COMPARISON OF LIPIDS AND LIPOPOLYSACCHARIDE FROM THE BACILLARY AND L FORMS OF PROTEUS P18. J Bacteriol. 1965 Apr;89:1020–1025. doi: 10.1128/jb.89.4.1020-1025.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Oró J., Tornabene T. G., Nooner D. W., Gelpi E. Aliphatic hydrocarbons and fatty acids of some marine and freshwater microorganisms. J Bacteriol. 1967 Jun;93(6):1811–1818. doi: 10.1128/jb.93.6.1811-1818.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Tornabene T. G., Oró J. 14-C incorporation into the fatty acids and aliphatic hydrocarbons of Sarcina lutea. J Bacteriol. 1967 Aug;94(2):349–358. doi: 10.1128/jb.94.2.349-358.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES