Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1968 Jun;95(6):2078–2082. doi: 10.1128/jb.95.6.2078-2082.1968

Methylation of the Cellular Lipid of Methionine-requiring Agrobacterium tumefaciens

Tsuneo Kaneshiro 1
PMCID: PMC315137  PMID: 5669890

Abstract

Mutants of Agrobacterium tumefaciens requiring methionine for growth on a solid basal medium were induced by the use of N-methyl-N′-nitro-N-nitrosoguanidine. In addition to the difference of mutant strains, the extent of methionine dependency differed in a liquid basal medium and in the presence of aspartate or fumarate. When (14C-methyl)-methionine was added to strain WM-11 growing in a prescribed basal medium, incorporation of 14C into the cellular “residue” fraction and polar “N-methylated” lipid fraction depended strictly on cellular growth and on external methionine concentration. However, a net synthesis of the “cyclopropane” fatty acid fraction occurred even during the maximal stationary phase if excess methionine was present.

Full text

PDF
2082

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. GOLDFINE H., ELLIS M. E. N-METHYL GROUPS IN BACTERIAL LIPIDS. J Bacteriol. 1964 Jan;87:8–15. doi: 10.1128/jb.87.1.8-15.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. GUEST J. R., HELLEINER C. W., CROSS M. J., WOODS D. D. Cobalamin and the synthesis of methionine by ultrasonic extracts of Escherichia coli. Biochem J. 1960 Aug;76:396–405. doi: 10.1042/bj0760396. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. HILDEBRAND J. G., LAW J. H. FATTY ACID DISTRIBUTION IN BACTERIAL PHOSPHOLIPIDS. THE SPECIFICITY OF THE CYCLOPROPANE SYNTHETASE REACTION. Biochemistry. 1964 Sep;3:1304–1308. doi: 10.1021/bi00897a020. [DOI] [PubMed] [Google Scholar]
  5. Hagen P. O., Goldfine H., Williams P. J. Phospholipids of bacteria with extensive intracytoplasmic membranes. Science. 1966 Mar 25;151(3717):1543–1544. doi: 10.1126/science.151.3717.1543. [DOI] [PubMed] [Google Scholar]
  6. Karkas J. D., Türler H., Chargaff E. Studies on the specification of accessory biochemical characters, as exemplified by the fatty acid patterns of various strains of Escherichia coli. Biochim Biophys Acta. 1965 Nov 15;111(1):96–109. doi: 10.1016/0304-4165(65)90476-9. [DOI] [PubMed] [Google Scholar]
  7. LEDERBERG J., LEDERBERG E. M. Replica plating and indirect selection of bacterial mutants. J Bacteriol. 1952 Mar;63(3):399–406. doi: 10.1128/jb.63.3.399-406.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LIU T. Y., HOFMANN K. Cyclopropane ring blosynthesis. Biochemistry. 1962 Jan;1:189–191. doi: 10.1021/bi00907a028. [DOI] [PubMed] [Google Scholar]
  9. POHL S., LAW J. H., RYHAGE R. THE PATH OF HYDROGEN IN THE FORMATION OF CYCLOPROPANE FATTY ACIDS. Biochim Biophys Acta. 1963 Oct 22;70:583–585. doi: 10.1016/0006-3002(63)90794-7. [DOI] [PubMed] [Google Scholar]
  10. SHAPIRO S. K. Utilization of S-adenosylmethionine by micro-organisms. J Bacteriol. 1962 Jan;83:169–174. doi: 10.1128/jb.83.1.169-174.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. STADTMAN E. R., COHEN G. N., LE BRAS G., de ROBICHON-SZULMAJSTER Selective feedback inhibition and repression of two aspartokinases in the metabolism of Escherichia coli. Cold Spring Harb Symp Quant Biol. 1961;26:319–321. doi: 10.1101/sqb.1961.026.01.038. [DOI] [PubMed] [Google Scholar]
  12. Scarborough G. A., Nyc J. F. Methylation of ethanolamine phosphatides by microsomes from normal and mutant strains of Neurospora crassa. J Biol Chem. 1967 Jan 25;242(2):238–242. [PubMed] [Google Scholar]
  13. Scarborough G. A., Nyc J. F. Properties of a phosphatidylmonomethylethanolamine N-methyl-transferase from Neurospora crassa. Biochim Biophys Acta. 1967 Sep 12;146(1):111–119. doi: 10.1016/0005-2744(67)90077-0. [DOI] [PubMed] [Google Scholar]
  14. Sherr S. I., Law J. H. Phosphatidylcholine synthesis in Agrobacterium tumefaciens. II. Uptake and utilization of choline. J Biol Chem. 1965 Oct;240(10):3760–3765. [PubMed] [Google Scholar]
  15. Silbert D. F., Vagelos P. R. Fatty acid mutant of E. coli lacking a beta-hydroxydecanoyl thioester dehydrase. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1579–1586. doi: 10.1073/pnas.58.4.1579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Starr M. P. Nutrition of Phytopathogenic Bacteria: I. Minimal Nutritive Requirements of Genus Xanthomonas. J Bacteriol. 1946 Feb;51(2):131–143. [PMC free article] [PubMed] [Google Scholar]
  17. Starr M. P. The Nutrition of Phytopathogenic Bacteria: II. The Genus Agrobacterium. J Bacteriol. 1946 Aug;52(2):187–194. [PMC free article] [PubMed] [Google Scholar]
  18. Weissbach H., Taylor R. Role of vitamin B12 in methionine synthesis. Fed Proc. 1966 Nov-Dec;25(6):1649–1656. [PubMed] [Google Scholar]
  19. ZALKIN H., LAW J. H., GOLDFINE H. Enzymatic synthesis of cyclopropane fatty acids catalyzed by bacterial extracts. J Biol Chem. 1963 Apr;238:1242–1248. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES