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. 1968 Jun;95(6):2393–2398. doi: 10.1128/jb.95.6.2393-2398.1968

Structural and Immunological Studies on the Protoplast Membrane of the Yeast Candida utilis

C Garcia Mendoza a, M D Garcia Lopez a,1, F Uruburu a,1, J R Villanueva a,1
PMCID: PMC315174  PMID: 5669909

Abstract

Cell membranes of the yeast Candida utilis isolated by lysis of protoplasts have been shown to be lipoprotein in nature. Electron microscopy shows that Mg++ is responsible for maintaining the integrity of the membrane. A close serological relationship was found between membranes and cell walls isolated from the yeast. This relationship was exhibited not only by membranes obtained by strepzyme treatment but also by those obtained from the action of helicase enzyme. No such relationship existed between membranes and whole cells. Related data have been obtained by treatment of yeasts with different digestive enzymes. All of the results suggest that the protoplast membrane possesses traces of structural cell wall material. This material is detectable by serological tests, but not by electron microscopy.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOULTON A. A. SOME OBSERVATIONS ON THE CHEMISTRY AND MORPHOLOGY OF THE MEMBRANES RELEASED FROM YEAST PROTOPLASTS BY OSMOTIC SHOCK. Exp Cell Res. 1965 Feb;37:343–359. doi: 10.1016/0014-4827(65)90183-7. [DOI] [PubMed] [Google Scholar]
  2. BOYDELATOUR E., BOLLE A., KELLENBERGER E. NOUVELLES OBSERVATIONS CONCERNANT L'ACTION DU LAURYLSULFATE DE SODIUM SUR LA PAROI ET LA MEMBRAND D'E. COLI. Pathol Microbiol (Basel) 1965;28:229–234. [PubMed] [Google Scholar]
  3. GASCON S., OCHOA A. G., VILLANUEVA J. R. PRODUCTION OF YEAST AND MOLD PROTOPLASTS BY TREATMENT WITH THE STREPZYME OF MICROMONOSPORA AS. Can J Microbiol. 1965 Jun;11:573–580. doi: 10.1139/m65-074. [DOI] [PubMed] [Google Scholar]
  4. MENDOZA C. G., VILLANUEVA J. R. STAGES IN THE FORMATION OF YEAST PROTOPLASTS WITH STREPZYME. Nature. 1964 Jun 20;202:1241–1242. doi: 10.1038/2021241a0. [DOI] [PubMed] [Google Scholar]
  5. MUNDKUR B. Electron microscopical studies of frozen-dried yeast. I. Localization of polysaccharides. Exp Cell Res. 1960 Jun;20:28–42. doi: 10.1016/0014-4827(60)90219-6. [DOI] [PubMed] [Google Scholar]
  6. Matile P., Moor H., Mühlethaler K. Isolation and properties of the plasmalemma in yeast. Arch Mikrobiol. 1967;58(3):201–211. doi: 10.1007/BF00408804. [DOI] [PubMed] [Google Scholar]
  7. Mendoza C. G., Villanueva J. R. Preparation and composition of the protoplast membrane of Candida utilis. Biochim Biophys Acta. 1967 May 2;135(2):189–195. doi: 10.1016/0005-2736(67)90113-7. [DOI] [PubMed] [Google Scholar]
  8. NOVAES M. QUELQUES DONN'EES SUR LA COMPOSITION CHIMIQUE DES PAROIS DE CANDIDA UTILIS. Biochim Biophys Acta. 1963 Dec 13;78:797–799. doi: 10.1016/0006-3002(63)91066-7. [DOI] [PubMed] [Google Scholar]
  9. Ottolenghi P. Do yeasts form true protoplasts? C R Trav Lab Carlsberg. 1966;35(15):363–368. [PubMed] [Google Scholar]
  10. REESE E. T., PARRISH F. W., MANDELS M. Beta-d-1, 6-Glucanases in fungi. Can J Microbiol. 1962 Jun;8:327–334. doi: 10.1139/m62-045. [DOI] [PubMed] [Google Scholar]

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