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. 1969 Jun;98(3):888–897. doi: 10.1128/jb.98.3.888-897.1969

Induction by Mercuric Ion of Extensive Degradation of Cellular Ribonucleic Acid in Escherichia coli

Teruhiko Beppu 1, Kei Arima 1
PMCID: PMC315269  PMID: 4892383

Abstract

Low concentrations of HgCl2 were found to induce extensive degradation of ribonucleic acid (RNA) in exponentially growing Escherichia coli cells but not in stationary-phase cells. Whereas 80% of cellular RNA was degraded during 90 min of incubation with 10−5m HgCl2 at 37 C, HgCl2 caused only slight degradation in stationary cells, even when present at concentrations higher than 5 × 10−5m. Inhibition of RNA synthesis occurred at almost the same concentration of HgCl2 as degradation, and the ability of stationary-phase cells to synthesize RNA was also resistant to HgCl2. The transition of cells from complete sensitivity to HgCl2 to a fully insensitive state took place simultaneously with the cessation of growth. p-Chloromercuribenzoate was also found to induce remarkable degradation of RNA. In E. coli Q13, a mutant deficient for ribonuclease I, no degradation of RNA was evident, even in the exponential growth phase. 3′-Mononucleotides but not 5′-mononucleotides were found among the degradation products of cellular RNA. 2′,3′-Cyclic mononucleotides were produced when RNA was degraded by the cell-free extracts of the Hg treated cells. Almost complete unmasking of the latent ribonuclease occurred in the particle fraction containing subribosomal particles of the Hg-treated cells. These data suggest that the incubation of exponentially growing E. coli cells with HgCl2 led to the unmasking of ribonuclease I, which resulted in the extensive degradation of cellular RNA. The activation of ribonuclease by HgCl2 in the isolated particulate fraction of E. coli K-12 which occurred in vitro suggested the presence of an Hg-sensitive inhibitor for ribonuclease I.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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