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. 1969 Oct;100(1):269–275. doi: 10.1128/jb.100.1.269-275.1969

Evolution of a Site of Specific Genetic Homology on the Chromosome of Escherichia coli

Phyllis L Kahn 1
PMCID: PMC315388  PMID: 4898993

Abstract

Integration of the factors Fv and F into the chromosome of a substrain of Escherichia coli K-12 has been studied. The Fv factor is a fertility factor derived from Col V, lacking the ability to govern the production of colicin V. The derivatives of an Hfrv (Hfr isolated from a V colicinogenic parent) strain, PK2 (initially isolated from C600 V+), were shown to retain a unique bidirectional sex factor affinity locus between recA and pheA. This site shows no affinity for the E. coli K-12 F factor as shown by inability to isolate Hfr strains with origins in this region from a parental strain containing a cytoplasmic F factor. However this area exhibits two regions of homology to the V colicinogenic factor. One gives rise to Hfrv strains identical to the original Hfrv strain, PK2, with an origin and polarity of transfer designated pheA-CC injecting markers in the order pheA-his-trp-pro. The second gives rise to strains apparently originating at the same site but with reverse polarity designated recA-C, transferring markers in the order recA-thyA-str-xyl. For strains possessing the Fv factor only the second homology is apparent. A model for the evolution of these strains is presented.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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