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. 1969 Oct;100(1):365–369. doi: 10.1128/jb.100.1.365-369.1969

Accumulation of Sulfite by a Sulfate-Using Revertant of Salmonella pullorum1

Bruce C Kline a,2, Delbert E Schoenhard a
PMCID: PMC315401  PMID: 5344100

Abstract

Sulfate-utilizing revertants of a cysteine auxotroph of Salmonella pullorum have been found that cause an accumulation in growth medium of a sulfur compound more oxidized than sulfide. The sulfur compound was presumptively identified as sulfite by the formation of a colored complex in the presence of basic fuchsin and formaldehyde, oxidation to sulfate by peroxide, and acid-volatility. The acid-volatile material was identified as sulfite by formation of an S-sulfonyl derivative of 5,5′-dithio-bis(2-nitrobenzoic acid) which was chromatographically and electrophoretically identical to an authentic S-sulfonyl derivative. The presence of sulfate in minimal medium is required for sulfite accumulation, and both cysteine and selenate inhibit the accumulation. No evidence was obtained to indicate that a reduced sulfur compound was the precursor of the accumulated sulfite.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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