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. 1965 Aug;90(2):403–410. doi: 10.1128/jb.90.2.403-410.1965

Accumulation of Ribonucleoprotein Particles in a Relaxed Mutant of Escherichia coli

Paul S Sypherd a,1
PMCID: PMC315658  PMID: 14329453

Abstract

Sypherd, Paul S. (University of California, San Diego). Accumulation of ribonucleoprotein particles in a relaxed mutant of Escherichia coli. J. Bacteriol. 90:403–410. 1965.—The synthesis of ribonucleic acid during amino acid deprivation of a “relaxed” mutant was investigated. Aspects of the stability of the macromolecular ribonucleic acid (RNA) were studied, and standard conditions were established to allow maximal recovery of the larger RNA's (i.e., 16S and 23S). These RNA's, representing 75% of the total RNA produced during starvation, were present in particles with nominal S-values of 20, 30, and 43. The particles are extremely sensitive to nuclease action, being completely destroyed in the presence of 2 μg/ml of pancreatic ribonuclease at 15 C for 30 min. The particles containing the bulk of the RNA were shown to be ribonucleoprotein, consisting of 26 to 28% protein by weight. It was shown that no mature 70S ribosomes were formed during the accumulation of the lighter, protein-deficient particles.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOREK E., RYAN A., ROCKENBACH J. Nucleic acid metabolism in relation to the lysogenic phenomenon. J Bacteriol. 1955 Apr;69(4):460–467. doi: 10.1128/jb.69.4.460-467.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DAGLEY S., TURNOCK G., WILD D. G. THE ACCUMULATION OF RIBONUCLEIC ACID BY A MUTANT OF ESCHERICHIA COLI. Biochem J. 1963 Sep;88:555–566. doi: 10.1042/bj0880555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. KIRBY K. S. A new method for the isolation of ribonucleic acids from mammalian tissues. Biochem J. 1956 Nov;64(3):405–408. doi: 10.1042/bj0640405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. MCCARTHY B. J., ARONSON A. I. The kinetics of the synthesis of ribosomal RNA in E. coli. Biophys J. 1961 Jan;1:227–245. doi: 10.1016/s0006-3495(61)86886-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. NEIDHARDT F. C., EIDLIC L. Characterization of the RNA formed under conditions of relaxed amino acid control in Escherichia coli. Biochim Biophys Acta. 1963 Mar 26;68:380–388. doi: 10.1016/0006-3002(63)90159-8. [DOI] [PubMed] [Google Scholar]
  7. NEIDHARDT F. C. Properties of a bacterial mutant lacking amino acid control of RNA synthesis. Biochim Biophys Acta. 1963 Mar 26;68:365–379. doi: 10.1016/0006-3002(63)90158-6. [DOI] [PubMed] [Google Scholar]
  8. SPAHR P. F. Amino acid composition of ribosomes from Escherichia coli. J Mol Biol. 1962 May;4:395–406. doi: 10.1016/s0022-2836(62)80020-5. [DOI] [PubMed] [Google Scholar]
  9. STENT G. S., BRENNER S. A genetic locus for the regulation of ribonucleic acid synthesis. Proc Natl Acad Sci U S A. 1961 Dec 15;47:2005–2014. doi: 10.1073/pnas.47.12.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. SYPHERD P. S. FORMATION OF RIBOSOMES FROM PRECURSOR RIBONUCLEOPROTEIN PARTICLES. J Bacteriol. 1965 Aug;90:411–417. doi: 10.1128/jb.90.2.411-417.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sypherd P. S., Strauss N. CHLORAMPHENICOL-PROMOTED REPRESSION OF beta-GALACTOSIDASE SYNTHESIS IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1963 Mar;49(3):400–407. doi: 10.1073/pnas.49.3.400. [DOI] [PMC free article] [PubMed] [Google Scholar]

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