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. 1965 Sep;90(3):681–686. doi: 10.1128/jb.90.3.681-686.1965

Nonphotoreactivating Repair of Ultraviolet Light-Damaged Transforming Deoxyribonucleic Acid by Micrococcus lysodeikticus Extracts1

Robert L Elder a,2, Roland F Beers Jr a
PMCID: PMC315710  PMID: 16562067

Abstract

Elder, Robert L. (Johns Hopkins University, Baltimore, Md.), and Roland F. Beers, Jr. Nonphotoreactivating repair of ultraviolet light-damaged transforming deoxyribonucleic acid by Micrococcus lysodeikticus extracts. J. Bacteriol. 90:681–686. 1965.—Extracts from Micrococcus lysodeikticus repair Haemophilus influenzae transforming deoxyribonucleic acid (DNA) damaged by ultraviolet light radiation. The repair is demonstrable over a wide dose range, with a constant dose reduction factor for a given concentration of DNA. The active component in the crude extract may be separated into a heat-stable dialyzable and a heat-labile nondialyzable component. The dialyzable fraction contains at least one component which appears to limit the maximal level of repair. Mg2+ ions are required for the repair process.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEERS R. F., Jr Polynucleotides; partial purification and properties of a polynucleotide phosphorylase from Micrococcus lysodeikticus. Biochem J. 1957 Aug;66(4):686–693. doi: 10.1042/bj0660686. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BOYCE R. P., HOWARD-FLANDERS P. RELEASE OF ULTRAVIOLET LIGHT-INDUCED THYMINE DIMERS FROM DNA IN E. COLI K-12. Proc Natl Acad Sci U S A. 1964 Feb;51:293–300. doi: 10.1073/pnas.51.2.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. ELDER R. L., BEERS R. F., Jr NONPHOTOREACTIVATING REPAIR OF ULTRAVIOLET LIGHT-DAMAGED MICROCOCCUS LYSODEIKTICUS CELLS. J Bacteriol. 1965 May;89:1225–1230. doi: 10.1128/jb.89.5.1225-1230.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. GOODGAL S. H., HERRIOTT R. M. Studies on transformations of Hemophilus influenzae. I. Competence. J Gen Physiol. 1961 Jul;44:1201–1227. doi: 10.1085/jgp.44.6.1201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. KELNER A. Growth, respiration, and nucleic acid synthesis in ultraviolet-irradiated and in photoreactivated Escherichia coli. J Bacteriol. 1953 Mar;65(3):252–262. doi: 10.1128/jb.65.3.252-262.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kelner A. Effect of Visible Light on the Recovery of Streptomyces Griseus Conidia from Ultra-violet Irradiation Injury. Proc Natl Acad Sci U S A. 1949 Feb;35(2):73–79. doi: 10.1073/pnas.35.2.73. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. ROERSCH A., VAN DER KAM P. C., ADEMA J. DARK REACTIVATION OF ULTRAVIOLET IRRADIATED BACTERIOPHAGE DEOXYRIBONUCLEIC ACID IN VITRO. Biochim Biophys Acta. 1964 Feb 17;80:346–348. [PubMed] [Google Scholar]
  8. RUPERT C. S. Photoenzymatic repair of ultraviolet damage in DNA. I. Kinetics of the reaction. J Gen Physiol. 1962 Mar;45:703–724. doi: 10.1085/jgp.45.4.703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. RUPERT C. S. Photoenzymatic repair of ultraviolet damage in DNA. II. Formation of an enzyme-substrate complex. J Gen Physiol. 1962 Mar;45:725–741. doi: 10.1085/jgp.45.4.725. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. RUPERT C. S. Photoreactivation of transforming DNA by an enzyme from bakers' yeast. J Gen Physiol. 1960 Jan;43:573–595. doi: 10.1085/jgp.43.3.573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. SETLOW R. B., CARRIER W. L. THE DISAPPEARANCE OF THYMINE DIMERS FROM DNA: AN ERROR-CORRECTING MECHANISM. Proc Natl Acad Sci U S A. 1964 Feb;51:226–231. doi: 10.1073/pnas.51.2.226. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SETLOW R. B., SETLOW J. K. Evidence that ultraviolet-induced thymine dimers in DNA cause biological damage. Proc Natl Acad Sci U S A. 1962 Jul 15;48:1250–1257. doi: 10.1073/pnas.48.7.1250. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. WULFF D. L., RUPERT C. S. Disappearance of thymine photodimer in ultraviolet irradiated DNA upon treatment with a photoreactivating enzyme from baker's yeast. Biochem Biophys Res Commun. 1962 Apr 20;7:237–240. doi: 10.1016/0006-291x(62)90181-x. [DOI] [PubMed] [Google Scholar]

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