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. 1965 Nov;90(5):1467–1480. doi: 10.1128/jb.90.5.1467-1480.1965

Structure of Bacterial L Forms and Their Parent Bacteria

Claes Weibull a,1
PMCID: PMC315836  PMID: 5848335

Abstract

Weibull, Claes (Rocky Mountain Laboratory, Hamilton, Mont.). Structure of bacterial L forms and their parent bacteria. J. Bacteriol. 90:1467–1480. 1965.—Light and electron microscopic studies were done on normal cells and L forms of Proteus mirabilis, Staphylococcus aureus, and Corynebacterium sp. grown in liquid media. Under the prevailing growth conditions, the L forms studied were morphologically indistinguishable from one another. They appeared as approximately spherical elements occurring singly or more often connected with each other by thinner portions of cell material. In sections of large L forms, the following structures were seen: a peripheral, triple-layered (“unit”) membrane, a granular cytoplasm, nuclear regions, and vacuoles limited by membranes. Small bodies often were present inside the vacuoles. These bodies also contained a peripheral membrane and a granular cytoplasm but usually no nuclear regions. The normal bacteria from which the L forms were derived differed markedly in structure from one another, especially in the surface layers of the cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABRAMS R. Y. A method for the cultivation of L forms in liquid media. J Bacteriol. 1955 Aug;70(2):251–251. doi: 10.1128/jb.70.2.251-251.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CLARKE P. H., LILLY M. D. A general structure for cell walls of gram-negative bacteria. Nature. 1962 Aug 4;195:517–518. doi: 10.1038/195516b0. [DOI] [PubMed] [Google Scholar]
  3. DIENES L., SHARP J. T. The role of high electrolyte concentration in the production and growth of L forms of bacteria. J Bacteriol. 1956 Feb;71(2):208–213. doi: 10.1128/jb.71.2.208-213.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DIENES L., WEINBERGER H. J. The L forms of bacteria. Bacteriol Rev. 1951 Dec;15(4):245–288. doi: 10.1128/br.15.4.245-288.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DOMERMUTH C. H., NIELSEN M. H., FREUNDT E. A., BIRCH-ANDERSEN A. ULTRASTRUCTURE OF MYCOPLASMA SPECIES. J Bacteriol. 1964 Sep;88:727–744. doi: 10.1128/jb.88.3.727-744.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. KELLENBERGER E., RYTER A. Cell wall and cytoplasmic membrane of Escherichia coli. J Biophys Biochem Cytol. 1958 May 25;4(3):323–326. doi: 10.1083/jcb.4.3.323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MORRISON T. H., WEIBULL C. The occurrence of cell wall constituents in stable Proteus L forms. Acta Pathol Microbiol Scand. 1962;55:475–482. doi: 10.1111/j.1699-0463.1962.tb04153.x. [DOI] [PubMed] [Google Scholar]
  8. MURRAY R. G., STEED P., ELSON H. E. THE LOCATION OF THE MUCOPEPTIDE IN SECTIONS OF THE CELL WALL OF ESCHERICHIA COLI AND OTHER GRAM-NEGATIVE BACTERIA. Can J Microbiol. 1965 Jun;11:547–560. doi: 10.1139/m65-072. [DOI] [PubMed] [Google Scholar]
  9. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. ROBERTSON J. D. The ultrastructure of cell membranes and their derivatives. Biochem Soc Symp. 1959;16:3–43. [PubMed] [Google Scholar]
  11. RYTER A., LANDMAN O. E. ELECTRON MICROSCOPE STUDY OF THE RELATIONSHIP BETWEEN MESOSOME LOSS AND THE STABLE L STATE (OR PROTOPLAST STATE) IN BACILLUS SUBTILIS. J Bacteriol. 1964 Aug;88:457–467. doi: 10.1128/jb.88.2.457-467.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SUGANUMA A. THE PLASMA MEMBRANE OF STAPHYLOCOCCUS AUTREUS TREATED WITH HCL SOLUTIONS. J Cell Biol. 1964 May;21:290–293. doi: 10.1083/jcb.21.2.290. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. TAUBENECK U. Uber Plasmolyseversuche mit penicillininduzierten Umbildungsformen von Proteus vulgaris. Zentralbl Bakteriol Orig. 1955 Oct;163(7-8):477–483. [PubMed] [Google Scholar]
  14. TULASNE R., MINCK R., KIRN A. [Comparative electron microscope study of a Proteus and corresponding L forms of A and B types]. Ann Inst Pasteur (Paris) 1962 Mar;102:292–299. [PubMed] [Google Scholar]
  15. WEIBULL C. Chemical analyses elucidating the structure of bacterial L forms. Acta Pathol Microbiol Scand. 1958;42(4):324–332. doi: 10.1111/j.1699-0463.1958.tb01746.x. [DOI] [PubMed] [Google Scholar]
  16. WEIBULL C., GYLLANG H. METABOLIC PROPERTIES OF SOME L FORMS DERIVED FROM GRAM-POSITIVE AND GRAM-NEGATIVE BACTERIA. J Bacteriol. 1965 Jun;89:1443–1447. doi: 10.1128/jb.89.6.1443-1447.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. WEIBULL C., LUNDIN B. M. Morphology of pleuropneumonia-like organisms and bacterial L forms grown in liquid media. J Bacteriol. 1963 Feb;85:440–445. doi: 10.1128/jb.85.2.440-445.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. WEIBULL C., MOHRI T., AFZELIUS B. A. THE MORPHOLOGY AND FINE STRUCTURE OF SMALL PARTICLES PRESENT IN CULTURES OF A PROTEUS L FORM. J Ultrastruct Res. 1965 Feb;12:81–91. doi: 10.1016/s0022-5320(65)80007-7. [DOI] [PubMed] [Google Scholar]
  19. WEIBULL C., MOHRI T. FIXATION OF BACTERIAL L FORMS FOR ELECTRON MICROSCOPY. J Bacteriol. 1965 Mar;89:901–902. doi: 10.1128/jb.89.3.901-902.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. WEIBULL C. PLASMOLYSIS IN BACILLUS MEGATERIUM. J Bacteriol. 1965 Apr;89:1151–1154. doi: 10.1128/jb.89.4.1151-1154.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. WEIBULL C. Size of minimal reproductive units of bacterial L forms. Proc Soc Exp Biol Med. 1963 May;113:32–34. doi: 10.3181/00379727-113-28267. [DOI] [PubMed] [Google Scholar]
  22. ZAMORA A., BOJALIL L. F., BASTARRACHEA F. IMMUNOLOGICALLY ACTIVE POLYSACCHARIDES FROM NOCARDIA ASTEROIDES AND NOCARDIA BRASILIENSIS. J Bacteriol. 1963 Mar;85:549–555. doi: 10.1128/jb.85.3.549-555.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]

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