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. 1966 Jan;91(1):374–383. doi: 10.1128/jb.91.1.374-383.1966

Biological Properties of Streptococcal Cell-Wall Particles III. Dermonecrotic Reaction to Cell-Wall Mucopeptides

Essa M Abdulla 1, John H Schwab 1
PMCID: PMC315957  PMID: 4955252

Abstract

Abdulla, Essa M. (University of North Carolina School of Medicine, Chapel Hill), and John H. Schwab. Biological properties of streptococcal cell-wall particles. III. Dermonecrotic reaction to cell-wall mucopeptides. J. Bacteriol. 91:374–383. 1966.—Intradermal injection of rabbits and guinea pigs with mucopeptide suspensions produced an acute necrotic lesion which reached maximal severity within 24 hr and gradually subsided with scar formation. Necrosis was evident within 4 hr after injection of 100 μg, and an indurated area (10 × 10 mm) was produced with as little as 5.0 μg. Mucopeptides from six bacterial strains were studied. Comparison of cell walls and derived mucopeptides showed that the acute necrotic lesion tended to be more severe as the residual polysaccharide was decreased. Hyperimmunization with mucopeptide reduced the acute reaction, with evidence of immunological specificity. Incubation with lysozyme also modified the reaction in relation to extent of digestion. Toxicity was related to particle size, since extended sonic vibration decreased activity. Histological sections showed intense accumulations of polymorphonuclear leukocytes, along with altered collagen. A chronic nodular lesion appeared about 7 days after injection of the intact cell-wall fragments. In contrast to the acute necrotic reaction, this lesion was rarely produced by the mucopeptide separated from polysaccharide.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABDULLA E. M., SCHWAB J. H. IMMUNOLOGICAL PROPERTIES OF BACTERIAL CELL WALL MUCOPEPTIDES. Proc Soc Exp Biol Med. 1965 Feb;118:359–362. doi: 10.3181/00379727-118-29842. [DOI] [PubMed] [Google Scholar]
  2. BARKULIS S. S., SMITH C., BOLTRALIK J. J., HEYMANN H. STRUCTURE OF STREPTOCOCCAL CELL WALLS. IV. PURIFICATION AND PROPERTIES OF STREPTOCOCCAL PHAGE MURALYSIN. J Biol Chem. 1964 Dec;239:4027–4033. [PubMed] [Google Scholar]
  3. BOREL Y., SCHWARTZ R. INHIBITION OF IMMEDIATE AND DELAYED HYPERSENSITIVITY IN THE RABBIT BY 6-MERCAPTOPURINE. J Immunol. 1964 May;92:754–761. [PubMed] [Google Scholar]
  4. CINADER B., LAFFERTY K. J. Antibody as inhibitor of ribonuclease: the role of steric hindrance, aggregate formation, and specificity. Ann N Y Acad Sci. 1963 May 8;103:653–673. doi: 10.1111/j.1749-6632.1963.tb53725.x. [DOI] [PubMed] [Google Scholar]
  5. CROMARTIE W. J., SCHWAB J. H., CRADDOCK J. G. The effect of a toxic cellular component of group A streptococci on connective tissue. Am J Pathol. 1960 Jul;37:79–99. [PMC free article] [PubMed] [Google Scholar]
  6. KRAUSE R. M., MCCARTY M. Studies on the chemical structure of the streptococcal cell wall. I. The identification of a mucopeptide in the cell walls of groups A and A-variant streptococci. J Exp Med. 1961 Jul 1;114:127–140. doi: 10.1084/jem.114.1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LARSON C. L., RIBI E., MILNER K. C., LIEBERMAN J. E. A method for titrating endotoxic activity in the skin of rabbits. J Exp Med. 1960 Jan 1;111:1–20. doi: 10.1084/jem.111.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. PARK J. T., HANCOCK R. A fractionation procedure for studies of the synthesis of cell-wall mucopeptide and of other polymers in cells of Staphylococcus aureus. J Gen Microbiol. 1960 Feb;22:249–258. doi: 10.1099/00221287-22-1-249. [DOI] [PubMed] [Google Scholar]
  9. ROBERSON B. S., CROMARTIE W. J. Influence of the physical state of endotoxic preparations on dermal toxicity. J Bacteriol. 1962 Nov;84:882–887. doi: 10.1128/jb.84.5.882-887.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. ROBERSON B. S., SCHWAB J. H., CROMARTIE W. J. Relation of particle size of C polysaccharide complexes of group A streptococci to toxic effects on connective tissue. J Exp Med. 1960 Nov 1;112:751–764. doi: 10.1084/jem.112.5.751. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. ROBERSON B. S., SCHWAB J. H. Endotoxic properties associated with cell walls of group A streptococci. J Infect Dis. 1961 Jan-Feb;108:25–34. doi: 10.1093/infdis/108.1.25. [DOI] [PubMed] [Google Scholar]
  12. SALTON M. R. J., HORNE R. W. Studies of the bacterial cell wall. II. Methods of preparation and some properties of cell walls. Biochim Biophys Acta. 1951 Jul;7(2):177–197. doi: 10.1016/0006-3002(51)90017-0. [DOI] [PubMed] [Google Scholar]
  13. SCHWAB J. H. ANALYSIS OF THE EXPERIMENTAL LESION OF CONNECTIVE TISSUE PRODUCED BY A COMPLEX OF C POLYSACCHARIDE FROM GROUP A STREPTOCOCCI. II. INFLUENCE OF AGE AND HYPERSENSITIVITY. J Exp Med. 1964 Mar 1;119:401–408. doi: 10.1084/jem.119.3.401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. SCHWAB J. H., CROMARTIE W. J., ROBERSON B. S. Identification of a toxic cellular component of group A streptococci as a complex of group-specific C polysaccharide and a protein. J Exp Med. 1959 Jan 1;109(1):43–54. doi: 10.1084/jem.109.1.43. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. SCHWAB J. H., CROMARTIE W. J. Studies on a toxic cellular component of group A streptococci. J Bacteriol. 1957 Nov;74(5):673–679. doi: 10.1128/jb.74.5.673-679.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. SCHWAB J. H., GOODER H., MAXTED W. R. Further studies on toxic C polysaccharide complexes of the beta-haemolytic streptococci. Br J Exp Pathol. 1962 Apr;43:181–188. [PMC free article] [PubMed] [Google Scholar]
  17. STETSON C. A., Jr Studies on the mechanism of the Shwartzman phenomenon; similarities between reactions to endotoxins and certain reactions of bacterial allergy. J Exp Med. 1955 Apr 1;101(4):421–436. doi: 10.1084/jem.101.4.421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schwab J. H. Biological properties of streptococcal cell-wall particles. I. Determinants of the chronic nodular lesion of connective tissue. J Bacteriol. 1965 Nov;90(5):1405–1411. doi: 10.1128/jb.90.5.1405-1411.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. WHEAT R. W., ROLLINS E. L., LEATHERWOOD J. M., BARNES R. L. Studies on the cell wall of Chromobacterium violaceum: the separation of lipopolysaccharide and mucopeptide by phenol extraction of whole cells. J Biol Chem. 1963 Jan;238:26–29. [PubMed] [Google Scholar]

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