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. 1966 Mar;91(3):1320–1326. doi: 10.1128/jb.91.3.1320-1326.1966

Isolation of Candida Protoplasts from a Case of Candida Endocarditis

Richard Rosner 1
PMCID: PMC316031  PMID: 4160231

Abstract

Rosner, Richard (St. Joseph's Hospital, Paterson, N.J.). Isolation of Candida protoplasts from a case of Candida endocarditis. J. Bacteriol. 91:1320–1326. 1966.—A case of endocarditis caused by Candida tropicalis is described. Even though the patient was receiving adequate therapy, and all routine blood cultures were negative for growth, the patient continued to give clinical evidence of active, progressive endocarditis. The isolation of osmotically fragile bodies from blood cultures placed in an osmotically controlled medium is described in detail. The role of these bodies, called protoplasts, in the active disease process of this patient is discussed in relation to the criteria for the implication of protoplasts in the disease process. Several explanations as to what caused the in vivo formation of protoplasts of C. tropicalis in this patient are discussed.

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Selected References

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  1. BACHMANN B. J., BONNER D. M. Protoplasts from Neurospora crassa. J Bacteriol. 1959 Oct;78:550–556. doi: 10.1128/jb.78.4.550-556.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DIENES L., WEINBERGER H. J. The L forms of bacteria. Bacteriol Rev. 1951 Dec;15(4):245–288. doi: 10.1128/br.15.4.245-288.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. HURWITZ C., REINER J. M., LANDAU J. V. Studies in the physiology and biochemistry of penicillin-induced spheroplasts of Escherichia coli. J Bacteriol. 1958 Dec;76(6):612–617. doi: 10.1128/jb.76.6.612-617.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. KINSKY S. C. Effect of polyene antibiotics on protoplasts of Neurospora crassa. J Bacteriol. 1962 Feb;83:351–358. doi: 10.1128/jb.83.2.351-358.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LANDMAN O. E., ALTENBERN R. A., GINOZA H. S. Quantitative conversion of cells and protoplasts of Proteus mirabilis and Escherichia coli to the L-form. J Bacteriol. 1958 May;75(5):567–576. doi: 10.1128/jb.75.5.567-576.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LEDERBERG J., ST CLAIR J. Protoplasts and L-type growth of Escherichia coli. J Bacteriol. 1958 Feb;75(2):143–160. doi: 10.1128/jb.75.2.143-160.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MICHAEL J. G., BRAUN W. Serum spheroplasts of Shigella dysenteriae. Proc Soc Exp Biol Med. 1959 Feb;100(2):422–425. doi: 10.3181/00379727-100-24649. [DOI] [PubMed] [Google Scholar]
  8. NECAS O. Regeneration of yeast cells from naked protoplasts. Nature. 1956 May 12;177(4515):898–899. doi: 10.1038/177898a0. [DOI] [PubMed] [Google Scholar]
  9. TULASNE R., MINCK R., KIRN A., KREMBEL J. [Delimitation of the concept of L forms of bacteria: protoplasts, spheroplasts and L forms]. Ann Inst Pasteur (Paris) 1960 Dec;99:859–874. [PubMed] [Google Scholar]
  10. WEIBULL C. Bacterial protoplasts. Annu Rev Microbiol. 1958;12:1–26. doi: 10.1146/annurev.mi.12.100158.000245. [DOI] [PubMed] [Google Scholar]

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