Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1966 Apr;91(4):1589–1593. doi: 10.1128/jb.91.4.1589-1593.1966

Quantitative Aspects of Exchangeable Calcium in Spores of Bacillus megaterium

L J Rode 1, J W Foster 1
PMCID: PMC316082  PMID: 4956344

Abstract

Rode, L. J. (The University of Texas, Austin), and J. W. Foster. Quantitative aspects of exchangeable calcium in spores of Bacillus megaterium. J. Bacteriol. 91:1589–1593. 1966.—More than 90% of the calcium in Ca45-labeled native spores was released from the cells during germination. Some 95% of the spore calcium was not exchangeable when ungerminated native spores were titrated to pH 4 with HNO3. Ca, Mg, Na, Si, and Fe were displaced from the spores by H+. The adsorption of Ca45 by H-spores and its subsequent release were studied under a variety of conditions. The isolated “coat fraction” of spores adsorbed substantial amounts of Ca45. Release of the adsorbed Ca was achieved with various reagents.

Full text

PDF
1593

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALDERTON G., SNELL N. Base exchange and heat resistance in bacterial spores. Biochem Biophys Res Commun. 1963 Jan 31;10:139–143. doi: 10.1016/0006-291x(63)90039-1. [DOI] [PubMed] [Google Scholar]
  2. ALDERTON G., THOMPSON P. A., SNELL N. HEAT ADAPTATION AND ION EXCHANGE IN BACILLUS MEGATERIUM SPORES. Science. 1964 Jan 10;143(3602):141–143. doi: 10.1126/science.143.3602.141. [DOI] [PubMed] [Google Scholar]
  3. Foerster H. F., Foster J. W. Endotrophic calcium, strontium, and barium spores of Bacillus megaterium and Bacillus cereus. J Bacteriol. 1966 Mar;91(3):1333–1345. doi: 10.1128/jb.91.3.1333-1345.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. KEYNAN A., HALVORSON H. O. Calcium dipicolinic acid-induced germination of Bacillus cereus spores. J Bacteriol. 1962 Jan;83:100–105. doi: 10.1128/jb.83.1.100-105.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. POWELL J. F. Factors affecting the germination of thick suspensions of bacillus subtilis spores in L-alanine solution. J Gen Microbiol. 1950 Sep;4(3):330–338. doi: 10.1099/00221287-4-3-330. [DOI] [PubMed] [Google Scholar]
  6. RODE L. J., FOSTER J. W. Germination of bacterial spores with alkyl primary amines. J Bacteriol. 1961 May;81:768–779. doi: 10.1128/jb.81.5.768-779.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. RODE L. J., FOSTER J. W. Ionic and non-ionic compounds in the germination of spores of Bacillus megaterium Texas. Arch Mikrobiol. 1962;43:201–212. doi: 10.1007/BF00406436. [DOI] [PubMed] [Google Scholar]
  8. Rode L. J., Foster J. W. Influence of exchangeable ions on germinability of bacterial spores. J Bacteriol. 1966 Apr;91(4):1582–1588. doi: 10.1128/jb.91.4.1582-1588.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rode L. J., Foster J. W. MECHANICAL GERMINATION OF BACTERIAL SPORES. Proc Natl Acad Sci U S A. 1960 Jan;46(1):118–128. doi: 10.1073/pnas.46.1.118. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES