Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1966 Jun;91(6):2158–2168. doi: 10.1128/jb.91.6.2158-2168.1966

Antigenic Properties of the Cell Wall and Other Fractions of the Yeast Form of Histoplasma capsulatum

Leo Pine 1, Clarence J Boone 1, Dave McLaughlin 1
PMCID: PMC316188  PMID: 5943933

Abstract

Pine, Leo (Communicable Disease Center, Atlanta, Ga.), Clarence J. Boone, and Dave McLaughlin. Antigenic properties of the cell wall and other fractions of the yeast form of Histoplasma capsulatum. J. Bacteriol. 91:2158–2168. 1966.—Yeast-form cells of Histoplasma capsulatum were fractionated in an attempt to obtain complement-fixing antigens with greater specificity than those of the currently used whole yeast form. No specific fraction was isolated. Rupture of the whole cell produced soluble and insoluble highly antigenic fractions. The soluble fractions rapidly lost antigenicity. Marked reduction of activity of some particulate fractions was also observed. The purified cell wall, stripped of its protein components, was antigenic, relatively stable, and demonstrated low cross-reactivity with heterologous sera. All fractions cross-reacted with sera from cases of North American blastomycosis to a greater degree than did the whole yeast-form antigen of H. capsulatum. The production of certain cellular fractions varied with the strains used. In complement-fixation tests, insignificant differences in specificity and antigenicity were found with whole-cell antigens from 13 H. capsulatum strains as well as with purified cell wall fractions prepared from 2 of these strains.

Full text

PDF
2168

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CAMPBELL C. C., BINKLEY G. E. Serologic diagnosis with respect to histoplasmosis, coccidioidomycosis, and blastomycosis and the problem of cross reactions. J Lab Clin Med. 1953 Dec;42(6):896–906. [PubMed] [Google Scholar]
  2. CAMPBELL C. C. The accuracy of serologic methods in diagnosis. Ann N Y Acad Sci. 1960 Aug 27;89:163–177. doi: 10.1111/j.1749-6632.1960.tb20139.x. [DOI] [PubMed] [Google Scholar]
  3. CUMMINS C. S., HARRIS H. The chemical composition of the cell wall in some gram-positive bacteria and its possible value as a taxonomic character. J Gen Microbiol. 1956 Jul;14(3):583–600. doi: 10.1099/00221287-14-3-583. [DOI] [PubMed] [Google Scholar]
  4. Campbell C. C. Reverting Histoplasma capsulatum to the Yeast Phase. J Bacteriol. 1947 Aug;54(2):263–264. [PMC free article] [PubMed] [Google Scholar]
  5. DISCHE Z. New color reactions for determination of sugars in polysaccharides. Methods Biochem Anal. 1955;2:313–358. doi: 10.1002/9780470110188.ch11. [DOI] [PubMed] [Google Scholar]
  6. KAUFMAN L., KAPLAN W. Preparation of a fluorescent antibody specific for the yeast phase of Histoplasma capsulatum. J Bacteriol. 1961 Nov;82:729–735. doi: 10.1128/jb.82.5.729-735.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LABZOFFSKY N. A., FISCHER J. B., HAMVAS J. J. Studies on the antigenic structure of Histoplasma capsulatum. Can J Microbiol. 1957 Dec;3(7):975–985. doi: 10.1139/m57-108. [DOI] [PubMed] [Google Scholar]
  8. MARKOWITZ H. POLYSACCHARIDE ANTIGENS FROM HISTOPLASMA CAPSULATUM. Proc Soc Exp Biol Med. 1964 Mar;115:697–700. doi: 10.3181/00379727-115-29010. [DOI] [PubMed] [Google Scholar]
  9. NORTHCOTE D. H., HORNE R. W. The chemical composition and structure of the yeast cell wall. Biochem J. 1952 May;51(2):232–236. doi: 10.1042/bj0510232. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. PARK J. T., JOHNSON M. J. A submicrodetermination of glucose. J Biol Chem. 1949 Nov;181(1):149–151. [PubMed] [Google Scholar]
  11. PINE L., DROUHET E. SUR L'OBTENTION ET LA CONSERVATION DE LA PHASE LEVURE D'HISTOPLASMA CAPSULATUM ET D'H. DUBOISI EN MILIEU CHIMIQUEMENT D'EFINI. Ann Inst Pasteur (Paris) 1963 Oct;105:798–804. [PubMed] [Google Scholar]
  12. PINE L., KAUFMAN L., BOONE C. J. COMPARATIVE FLUORESCENT ANTIBODY STAINING OF HISTOPLASMA CAPSULATUM AND HISTOPLASMA DUBOISII WITH A SPECIFIC ANTI-YEAST PHASE H. CAPSULATUM CONJUGATE. Mycopathol Mycol Appl. 1964 Dec 31;24:315–326. doi: 10.1007/BF02053642. [DOI] [PubMed] [Google Scholar]
  13. PINE L., PEACOCK C. L. Studies on the growth of Histoplasma capsulatum. IV. Factors influencing conversion of the mycelial phase to the yeast phase. J Bacteriol. 1958 Feb;75(2):167–174. doi: 10.1128/jb.75.2.167-174.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. PINE L. Studies on the growth of Histoplasma capsulatum. II. Growth of the yeast phase on agar media. J Bacteriol. 1955 Oct;70(4):375–381. doi: 10.1128/jb.70.4.375-381.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. ROWE J. R., NEWCOMER V. D., LANDAU J. W. EFFECTS OF CULTURAL CONDITIONS ON THE DEVELOPMENT OF ANTIGENS BY COCCIDIOIDES IMMITIS. J Invest Dermatol. 1963 Nov;41:343–350. doi: 10.1038/jid.1963.120. [DOI] [PubMed] [Google Scholar]
  16. SALVIN S. B., RIBI E. Antigens from yeast phase of Histoplasma capsulatum. II. Immunologic properties of protoplasm vs. cell walls. Proc Soc Exp Biol Med. 1955 Oct;90(1):287–294. doi: 10.3181/00379727-90-22012. [DOI] [PubMed] [Google Scholar]
  17. SCHUBERT J. H., AJELLO L., COOPER J. S., RUNYON L. C. Evaluation of histoplasmin and yeast phase antigens derived from a single strain of Histoplasma capsulatum in the complement fixation test. J Bacteriol. 1955 May;69(5):558–562. doi: 10.1128/jb.69.5.558-562.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. SCHUBERT J. H., AJELLO L. Variation in complement fixation antigenicity of different yeast phase strains of Histoplasma capsulatum. J Lab Clin Med. 1957 Aug;50(2):304–307. [PubMed] [Google Scholar]
  19. TROLL W., CANNAN R. K. A modified photometric ninhydrin method for the analysis of amino and imino acids. J Biol Chem. 1953 Feb;200(2):803–811. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES