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. 1966 Dec;92(6):1698–1705. doi: 10.1128/jb.92.6.1698-1705.1966

Tryptophan Transport in Neurospora crassa I. Specificity and Kinetics

W R Wiley 1, W H Matchett 1
PMCID: PMC316250  PMID: 5958105

Abstract

Wiley, W. R. (Battelle Memorial Institute, Richland, Wash.), and W. H. Matchett. Tryptophan transport in Neurospora crassa. I. Specificity and kinetics. J. Bacteriol. 92:1698–1705. 1966.—The transport of tryptophan in Neurospora crassa is mediated by a distinct stereospecific system which is chemically specific for a family of neutral amino acids. The process shows typical saturation kinetics and a sharp decrease in the rate of tryptophan uptake at low temperatures. The Q10 for the process is approximately 2 between 20 and 30 C. The apparent Km for uptake is 5 × 10−5m. Leucine and phenylalanine competitively inhibit the rate of tryptophan transport; the Ki values are 1.1 × 10−4m and 4.0 × 10−5m, respectively. These data are interpreted as evidence that these amino acids are transported by the same transport site(s). Inhibition studies with amino acids and other compounds structurally related to leucine and phenylalanine suggest that an uncharged side chain and an α amino group, next to a carboxyl, represent three attachment points for the uptake site.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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