Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1989 May 11;17(9):3469–3478. doi: 10.1093/nar/17.9.3469

Quantitative evaluation of Escherichia coli host strains for tolerance to cytosine methylation in plasmid and phage recombinants.

D M Woodcock 1, P J Crowther 1, J Doherty 1, S Jefferson 1, E DeCruz 1, M Noyer-Weidner 1, S S Smith 1, M Z Michael 1, M W Graham 1
PMCID: PMC317789  PMID: 2657660

Abstract

Many strains of E. coli K12 restrict DNA containing cytosine methylation such as that present in plant and animal genomes. Such restriction can severely inhibit the efficiency of cloning genomic DNAs. We have quantitatively evaluated a total of 39 E. coli strains for their tolerance to cytosine methylation in phage and plasmid cloning systems. Quantitative estimations of relative tolerance to methylation for these strains are presented, together with the evaluation of the most promising strains in practical recombinant cloning situations. Host strains are recommended for different recombinant cloning requirements. These data also provide a rational basis for future construction of 'ideal' hosts combining optimal methylation tolerance with additional advantageous mutations.

Full text

PDF
3472

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Casadaban M. J., Cohen S. N. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J Mol Biol. 1980 Apr;138(2):179–207. doi: 10.1016/0022-2836(80)90283-1. [DOI] [PubMed] [Google Scholar]
  2. Chalker A. F., Leach D. R., Lloyd R. G. Escherichia coli sbcC mutants permit stable propagation of DNA replicons containing a long palindrome. Gene. 1988 Nov 15;71(1):201–205. doi: 10.1016/0378-1119(88)90092-3. [DOI] [PubMed] [Google Scholar]
  3. Dila D., Raleigh E. A. Genetic dissection of the methylcytosine-specific restriction system mcrB of Escherichia coli K-12. Gene. 1988 Dec 25;74(1):23–24. doi: 10.1016/0378-1119(88)90241-7. [DOI] [PubMed] [Google Scholar]
  4. Fanning T. G., Singer M. F. LINE-1: a mammalian transposable element. Biochim Biophys Acta. 1987 Dec 8;910(3):203–212. doi: 10.1016/0167-4781(87)90112-6. [DOI] [PubMed] [Google Scholar]
  5. Heitman J., Model P. Site-specific methylases induce the SOS DNA repair response in Escherichia coli. J Bacteriol. 1987 Jul;169(7):3243–3250. doi: 10.1128/jb.169.7.3243-3250.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ishiura M., Ohashi H., Uchida T., Okada Y. Efficient simplified cosmid cloning: construction and characterization of cosmid vectors that carry the two cohesive end sites of lambda phages arrayed in tandem. Anal Biochem. 1989 Jan;176(1):117–127. doi: 10.1016/0003-2697(89)90281-9. [DOI] [PubMed] [Google Scholar]
  7. Noyer-Weidner M. A novel mcrB-based Escherichia coli K-12 vector system and its use in analyzing the genetic determinants for the McrB nuclease. Gene. 1988 Dec 25;74(1):177–178. doi: 10.1016/0378-1119(88)90279-x. [DOI] [PubMed] [Google Scholar]
  8. Noyer-Weidner M., Diaz R., Reiners L. Cytosine-specific DNA modification interferes with plasmid establishment in Escherichia coli K12: involvement of rglB. Mol Gen Genet. 1986 Dec;205(3):469–475. doi: 10.1007/BF00338084. [DOI] [PubMed] [Google Scholar]
  9. Raleigh E. A., Murray N. E., Revel H., Blumenthal R. M., Westaway D., Reith A. D., Rigby P. W., Elhai J., Hanahan D. McrA and McrB restriction phenotypes of some E. coli strains and implications for gene cloning. Nucleic Acids Res. 1988 Feb 25;16(4):1563–1575. doi: 10.1093/nar/16.4.1563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Raleigh E. A. Restriction and modification in vivo by Escherichia coli K12. Methods Enzymol. 1987;152:130–141. doi: 10.1016/0076-6879(87)52015-8. [DOI] [PubMed] [Google Scholar]
  11. Raleigh E. A., Wilson G. Escherichia coli K-12 restricts DNA containing 5-methylcytosine. Proc Natl Acad Sci U S A. 1986 Dec;83(23):9070–9074. doi: 10.1073/pnas.83.23.9070. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sain B., Murray N. E. The hsd (host specificity) genes of E. coli K 12. Mol Gen Genet. 1980;180(1):35–46. doi: 10.1007/BF00267350. [DOI] [PubMed] [Google Scholar]
  13. Shafit-Zagardo B., Brown F. L., Maio J. J., Adams J. W. KpnI families of long, interspersed repetitive DNAs associated with the human beta-globin gene cluster. Gene. 1982 Dec;20(3):397–407. doi: 10.1016/0378-1119(82)90208-6. [DOI] [PubMed] [Google Scholar]
  14. Smith S. S., Hardy T. A., Baker D. J. Human DNA (cytosine-5)methyltransferase selectively methylates duplex DNA containing mispairs. Nucleic Acids Res. 1987 Sep 11;15(17):6899–6916. doi: 10.1093/nar/15.17.6899. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sozhamannan S., Dharmalingam K. Molecular cloning and identification of protein products of rglB locus of Escherichia coli. Gene. 1988 Dec 25;74(1):51–52. doi: 10.1016/0378-1119(88)90249-1. [DOI] [PubMed] [Google Scholar]
  16. Whittaker P. A., Campbell A. J., Southern E. M., Murray N. E. Enhanced recovery and restriction mapping of DNA fragments cloned in a new lambda vector. Nucleic Acids Res. 1988 Jul 25;16(14B):6725–6736. doi: 10.1093/nar/16.14.6725. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wood W. B. Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA. J Mol Biol. 1966 Mar;16(1):118–133. doi: 10.1016/s0022-2836(66)80267-x. [DOI] [PubMed] [Google Scholar]
  18. Woodcock D. M., Crowther P. J., Diver W. P., Graham M., Bateman C., Baker D. J., Smith S. S. RglB facilitated cloning of highly methylated eukaryotic DNA: the human L1 transposon, plant DNA, and DNA methylated in vitro with human DNA methyltransferase. Nucleic Acids Res. 1988 May 25;16(10):4465–4482. doi: 10.1093/nar/16.10.4465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wyman A. R., Wertman K. F. Host strains that alleviate underrepresentation of specific sequences: overview. Methods Enzymol. 1987;152:173–180. doi: 10.1016/0076-6879(87)52017-1. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES