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. 1989 Sep 12;17(17):7029–7043. doi: 10.1093/nar/17.17.7029

Cloning of the complete Mycoplasma pneumoniae genome.

R Wenzel 1, R Herrmann 1
PMCID: PMC318431  PMID: 2506532

Abstract

The complete genome of Mycoplasma pneumoniae was cloned in an ordered library consisting of 34 overlapping or adjacent cosmids, one plasmid and two lambda phages. The genome size was determined by adding up the sizes of either the individual unique EcoRI restriction fragments of the gene bank or of the XhoI fragments of genomic M. pneumoniae DNA. The values from these calculations, 835 and 849 kbp, are in good agreement. An XhoI restriction map was constructed by identifying adjacent DNA fragments by probing with selected cosmid clones.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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