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. 1984 Mar 26;12(6):2851–2859. doi: 10.1093/nar/12.6.2851

The genes for the ribosomal proteins S12 and S7 are clustered with the gene for the EF-Tu protein on the chloroplast genome of Euglena gracilis.

P E Montandon, E Stutz
PMCID: PMC318710  PMID: 6324129

Abstract

We characterize a DNA segment of the Euglena gracilis chloroplast DNA fragment Eco . N by nucleotide sequencing and S1 nuclease analysis. We show that this region, which is upstream of the previously sequenced tuf A gene, contains the genes for the ribosomal proteins S12 and S7. The gene arrangement is 5'-rps 12-80 bp spacer-rps 7-174 bp spacer-tuf A, somewhat similar to the str operon of E. coli. The chloroplast S12 and S7 proteins contain 124 and 155 aminoacids, respectively, and are to 68% and 38% homologous with the corresponding E. coli proteins. The region is transcribed into a distronic mRNA of about 1.1 to 1.2 kb. The rps 12 and rps 7 genes, contrary to the tuf A gene, are not split.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berk A. J., Sharp P. A. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell. 1977 Nov;12(3):721–732. doi: 10.1016/0092-8674(77)90272-0. [DOI] [PubMed] [Google Scholar]
  2. Breitenberger C. A., Graves M. C., Spremulli L. L. Evidence for the nuclear location of the gene for chloroplast elongation factor G. Arch Biochem Biophys. 1979 Apr 15;194(1):265–270. doi: 10.1016/0003-9861(79)90617-9. [DOI] [PubMed] [Google Scholar]
  3. Favaloro J., Treisman R., Kamen R. Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping. Methods Enzymol. 1980;65(1):718–749. doi: 10.1016/s0076-6879(80)65070-8. [DOI] [PubMed] [Google Scholar]
  4. Funatsu G., Wittmann H. G. Ribosomal proteins. 33. Location of amino-acid replacements in protein S12 isolated from Escherichia coli mutants resistant to streptomycin. J Mol Biol. 1972 Jul 28;68(3):547–550. doi: 10.1016/0022-2836(72)90108-8. [DOI] [PubMed] [Google Scholar]
  5. Funatsu G., Yaguchi M., Wittmann-Liebold B. Primary stucture of protein S12 from the small Escherichia coli ribosomal subunit. FEBS Lett. 1977 Jan 15;73(1):12–17. doi: 10.1016/0014-5793(77)80004-5. [DOI] [PubMed] [Google Scholar]
  6. Graf L., Roux E., Stutz E., Kössel H. Nucleotide sequence of a Euglena gracilis chloroplast gene coding for the 16S rRNA: homologies to E. coli and Zea mays chloroplast 16S rRNA. Nucleic Acids Res. 1982 Oct 25;10(20):6369–6381. doi: 10.1093/nar/10.20.6369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jaskunas S. R., Lindahl L., Nomura M. Identification of two copies of the gene for the elongation factor EF-Tu in E. coli. Nature. 1975 Oct 9;257(5526):458–462. doi: 10.1038/257458a0. [DOI] [PubMed] [Google Scholar]
  8. Karabin G. D., Hallick R. B. Euglena gracilis chloroplast transfer RNA transcription units. Nucleotide sequence analysis of a tRNAThr-tRNAGly-tRNAMet-tRNASer-tRNAGln gene cluster. J Biol Chem. 1983 May 10;258(9):5512–5518. [PubMed] [Google Scholar]
  9. Messing J., Vieira J. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene. 1982 Oct;19(3):269–276. doi: 10.1016/0378-1119(82)90016-6. [DOI] [PubMed] [Google Scholar]
  10. Montandon P. E., Stutz E. Nucleotide sequence of a Euglena gracilis chloroplast genome region coding for the elongation factor Tu; evidence for a spliced mRNA. Nucleic Acids Res. 1983 Sep 10;11(17):5877–5892. doi: 10.1093/nar/11.17.5877. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nomura M., Mizushima S., Ozaki M., Traub P., Lowry C. V. Structure and function of ribosomes and their molecular components. Cold Spring Harb Symp Quant Biol. 1969;34:49–61. doi: 10.1101/sqb.1969.034.01.009. [DOI] [PubMed] [Google Scholar]
  12. Ortiz W., Reardon E. M., Price C. A. Preparation of chloroplasts from euglena highly active in protein synthesis. Plant Physiol. 1980 Aug;66(2):291–294. doi: 10.1104/pp.66.2.291. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Post L. E., Nomura M. DNA sequences from the str operon of Escherichia coli. J Biol Chem. 1980 May 25;255(10):4660–4666. [PubMed] [Google Scholar]
  14. Reinbolt J., Tritsch D. The primary structure of ribosomal protein S7 from E. coli strains K and B. FEBS Lett. 1978 Jul 15;91(2):297–301. doi: 10.1016/0014-5793(78)81196-x. [DOI] [PubMed] [Google Scholar]
  15. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  16. Rutti B., Keller M., Ortiz W., Stutz E. Analysis of Euglena gracilis chloroplast DNA. The DNA fragment EcoRI . N carries genetic information for a 53,000 M(r) polypeptide. FEBS Lett. 1981 Nov 2;134(1):15–19. doi: 10.1016/0014-5793(81)80540-6. [DOI] [PubMed] [Google Scholar]
  17. Sagher D., Grosfeld H., Edelman M. Large subunit ribulosebisphosphate carboxylase messenger RNA from Euglena chloroplasts. Proc Natl Acad Sci U S A. 1976 Mar;73(3):722–726. doi: 10.1073/pnas.73.3.722. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sanger F., Coulson A. R., Barrell B. G., Smith A. J., Roe B. A. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J Mol Biol. 1980 Oct 25;143(2):161–178. doi: 10.1016/0022-2836(80)90196-5. [DOI] [PubMed] [Google Scholar]
  19. Schwartzbach S. D., Schiff J. A. Chloroplast and cytoplasmic ribosomes of Euglena: selective binding of dihydrostreptomycin to chloroplast ribosomes. J Bacteriol. 1974 Oct;120(1):334–341. doi: 10.1128/jb.120.1.334-341.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wiegand R. C., Godson G. N., Radding C. M. Specificity of the S1 nuclease from Aspergillus oryzae. J Biol Chem. 1975 Nov 25;250(22):8848–8855. [PubMed] [Google Scholar]
  21. Yokota T., Sugisaki H., Takanami M., Kaziro Y. The nucleotide sequence of the cloned tufA gene of Escherichia coli. Gene. 1980 Dec;12(1-2):25–31. doi: 10.1016/0378-1119(80)90012-8. [DOI] [PubMed] [Google Scholar]

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